DiI Dye | AAT Bioquest

DiI iodide

1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide

DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiI and its analogs are most frequently used since they usually exhibit very low cell toxicity. In addition, DiI is widely used for determining lipoproteins such as LDL and HDL.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22101	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	104.024 µL	520.118 µL	1.04 mL	5.201 mL	10.402 mL
5 mM	20.805 µL	104.024 µL	208.047 µL	1.04 mL	2.08 mL
10 mM	10.402 µL	52.012 µL	104.024 µL	520.118 µL	1.04 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	961.32 g/mol	=	mL	x	mM	=	mmol

Citations

View all 53 citations: Citation Explorer

Engineering Central Substitutions in Heptamethine Dyes for Improved Fluorophore Performance

Authors:

Guo, Lei and Yang, Meek and Dong, Bin and Lewman, Seth and Van Horn, Alex and Jia, Shang

Journal:

JACS Au (2024)

Modular strategy for development of the hierarchical visual network in mice

Authors:

Murakami, Tomonari and Matsui, Teppei and Uemura, Masato and Ohki, Kenichi

Journal:

Nature (2022): 578--585

Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation

Authors:

Liu, Bowen and Jin, Yuan and Yang, Jingyi and Han, Yue and Shan, Hui and Qiu, Mantang and Zhao, Xuyang and Liu, Anhang and Jin, Yan and Yin, Yuxin

Journal:

Journal of Extracellular Vesicles (2022): e12223

A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer

Authors:

Liu, Lei and Pang, Honglin and He, Qiao and Pan, Biran and Sun, Xiaobin and Shan, Jing and Wu, Liping and Wu, Kaiwen and Yao, Xue and Guo, Yuanbiao

Journal:

Cancer Cell International (2021): 1--15

Insight into the in vivo translocation of oral liposomes by fluorescence resonance energy transfer effect

Authors:

Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping

Journal:

International Journal of Pharmaceutics (2020): 119682

References

View all 92 references: Citation Explorer

Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing

Authors:

Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.

Journal:

J Biomed Opt (2006): 50507

Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius

Authors:

Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.

Journal:

Hypertension (2006): 482

Functional neuroanatomy of the rhinophore of Aplysia punctata

Authors:

Wertz A, Rossler W, Obermayer M, Bickmeyer U.

Journal:

Front Zool (2006): 6

In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope

Authors:

Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.

Journal:

Invest Ophthalmol Vis Sci (2006): 2943

Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials

Authors:

Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.

Journal:

J Biomed Mater Res B Appl Biomater. (2006)

Physical properties

Molecular weight	961.32
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	148000
Excitation (nm)	550
Emission (nm)	564

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	41085-99-8

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Page updated on August 13, 2024