DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide]
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 961.32 |
Solvent | DMSO |
Spectral properties
Extinction coefficient (cm -1 M -1) | 148000 |
Excitation (nm) | 550 |
Emission (nm) | 564 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12352200 |
Overview | ![]() ![]() |
See also: Cell Signaling, Cell Structures and Organelles, Plasma Membrane, Cellular Processes, Membrane Potential and Channels, Physiological Probes
CAS 41085-99-8 | Molecular weight 961.32 | Extinction coefficient (cm -1 M -1) 148000 | Excitation (nm) 550 | Emission (nm) 564 |
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiI and its analogs are most frequently used since they usually exhibit very low cell toxicity. In addition, DiI is widely used for determining lipoproteins such as LDL and HDL.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 104.024 µL | 520.118 µL | 1.04 mL | 5.201 mL | 10.402 mL |
5 mM | 20.805 µL | 104.024 µL | 208.047 µL | 1.04 mL | 2.08 mL |
10 mM | 10.402 µL | 52.012 µL | 104.024 µL | 520.118 µL | 1.04 mL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 148000 |
Excitation (nm) | 550 |
Emission (nm) | 564 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Propidium iodide *CAS 25535-16-4* | 537 | 618 | 60001 |
Propidium iodide *10 mM aqueous solution* | 537 | 618 | 60001 |
DiR iodide [1,1-dioctadecyl-3,3,3,3-tetramethylindotricarbocyanine iodide] | 754 | 778 | 2700001 |
DiI perchlorate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate] *CAS 41085-99-8* | 550 | 564 | 148000 |
DiI triflate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine triflate] | 550 | 564 | 148000 |
Images
Citations
View all 52 citations: Citation Explorer
Extracellular vesicles from lung tissue drive bone marrow neutrophil recruitment in inflammation
Authors: Liu, Bowen and Jin, Yuan and Yang, Jingyi and Han, Yue and Shan, Hui and Qiu, Mantang and Zhao, Xuyang and Liu, Anhang and Jin, Yan and Yin, Yuxin
Journal: Journal of Extracellular Vesicles (2022): e12223
Authors: Liu, Bowen and Jin, Yuan and Yang, Jingyi and Han, Yue and Shan, Hui and Qiu, Mantang and Zhao, Xuyang and Liu, Anhang and Jin, Yan and Yin, Yuxin
Journal: Journal of Extracellular Vesicles (2022): e12223
Modular strategy for development of the hierarchical visual network in mice
Authors: Murakami, Tomonari and Matsui, Teppei and Uemura, Masato and Ohki, Kenichi
Journal: Nature (2022): 578--585
Authors: Murakami, Tomonari and Matsui, Teppei and Uemura, Masato and Ohki, Kenichi
Journal: Nature (2022): 578--585
A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer
Authors: Liu, Lei and Pang, Honglin and He, Qiao and Pan, Biran and Sun, Xiaobin and Shan, Jing and Wu, Liping and Wu, Kaiwen and Yao, Xue and Guo, Yuanbiao
Journal: Cancer Cell International (2021): 1--15
Authors: Liu, Lei and Pang, Honglin and He, Qiao and Pan, Biran and Sun, Xiaobin and Shan, Jing and Wu, Liping and Wu, Kaiwen and Yao, Xue and Guo, Yuanbiao
Journal: Cancer Cell International (2021): 1--15
Insight into the in vivo translocation of oral liposomes by fluorescence resonance energy transfer effect
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Radial extracorporeal shock wave promotes the enhanced permeability and retention effect to reinforce cancer nanothermotherapeutics
Authors: Yin, Chunyang and Wang, Shunhao and Ren, Quanzhong and Shen, Xinming and Chen, Xiaodong and Liu, Yajun and Liu, Sijin
Journal: Science Bulletin (2019)
Authors: Yin, Chunyang and Wang, Shunhao and Ren, Quanzhong and Shen, Xinming and Chen, Xiaodong and Liu, Yajun and Liu, Sijin
Journal: Science Bulletin (2019)
Loss of Integrity of Doxorubicin Liposomes during Transcellular Transportation Evidenced by Fluorescence Resonance Energy Transfer Effect
Authors: Li, Dong and Zhuang, Jie and Yang, Yinqian and Wang, D and an , undefined and Yang, Jinlong and He, Haisheng and Fan, Wufa and Banerjee, Amrita and Lu, Yi and Wu, Wei and others, undefined
Journal: Colloids and Surfaces B: Biointerfaces (2018)
Authors: Li, Dong and Zhuang, Jie and Yang, Yinqian and Wang, D and an , undefined and Yang, Jinlong and He, Haisheng and Fan, Wufa and Banerjee, Amrita and Lu, Yi and Wu, Wei and others, undefined
Journal: Colloids and Surfaces B: Biointerfaces (2018)
DOTA Functionalized Cross-Linked Small-Molecule Micelles for Theranostics Combining Magnetic Resonance Imaging and Chemotherapy
Authors: Feng, Jianyue and Luo, Qiang and Chen, Yun and Li, Bing and Luo, Kui and Lan, Jingbo and Yu, Yunlong and Zhang, Shiyong
Journal: Bioconjugate chemistry (2018)
Authors: Feng, Jianyue and Luo, Qiang and Chen, Yun and Li, Bing and Luo, Kui and Lan, Jingbo and Yu, Yunlong and Zhang, Shiyong
Journal: Bioconjugate chemistry (2018)
Decellularized corneal lenticule embedded compressed collagen: Toward a suturable collagenous construct for limbal reconstruction
Authors: Hong, Hyeonjun and Huh, Man-Il and Park, Sang Min and Lee, Kyoung-pil and Kim, Hong Kyun and Kim, Dong Sung
Journal: Biofabrication (2018)
Authors: Hong, Hyeonjun and Huh, Man-Il and Park, Sang Min and Lee, Kyoung-pil and Kim, Hong Kyun and Kim, Dong Sung
Journal: Biofabrication (2018)
Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
The accelerated blood clearance phenomenon of PEGylated nanoemulsion upon cross administration with nanoemulsions modified with polyglycerin
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
References
View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483
Application notes
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Non-Radioactive Photometric Assay for Glucose Uptake in Insulin-Responsive 3T3-L1 Adipocytes
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Non-Radioactive Photometric Assay for Glucose Uptake in Insulin-Responsive 3T3-L1 Adipocytes
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
Abbreviation of Common Chemical Compounds Related to Peptides