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DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide]

Chemical structure for DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide]
Chemical structure for DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide]
Ordering information
Price ()
Catalog Number22101
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight961.32
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)148000
Excitation (nm)550
Emission (nm)564
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
961.32
Extinction coefficient (cm -1 M -1)
148000
Excitation (nm)
550
Emission (nm)
564
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiI and its analogs are most frequently used since they usually exhibit very low cell toxicity. In addition, DiI is widely used for determining lipoproteins such as LDL and HDL.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiI iodide [1,1-Dioctadecyl-3,3,3,3- tetramethylindocarbocyanine iodide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM104.024 µL520.118 µL1.04 mL5.201 mL10.402 mL
5 mM20.805 µL104.024 µL208.047 µL1.04 mL2.08 mL
10 mM10.402 µL52.012 µL104.024 µL520.118 µL1.04 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)148000
Excitation (nm)550
Emission (nm)564

Citations


View all 50 citations: Citation Explorer
A novel strategy to identify candidate diagnostic and prognostic biomarkers for gastric cancer
Authors: Liu, Lei and Pang, Honglin and He, Qiao and Pan, Biran and Sun, Xiaobin and Shan, Jing and Wu, Liping and Wu, Kaiwen and Yao, Xue and Guo, Yuanbiao
Journal: Cancer Cell International (2021): 1--15
Insight into the in vivo translocation of oral liposomes by fluorescence resonance energy transfer effect
Authors: Liu, Wenjuan and Li, Dong and Dong, Zirong and Liu, Kaiheng and He, Haisheng and Lu, Yi and Wu, Wei and Li, Qinghua and Gan, Li and Qi, Jianping
Journal: International Journal of Pharmaceutics (2020): 119682
Radial extracorporeal shock wave promotes the enhanced permeability and retention effect to reinforce cancer nanothermotherapeutics
Authors: Yin, Chunyang and Wang, Shunhao and Ren, Quanzhong and Shen, Xinming and Chen, Xiaodong and Liu, Yajun and Liu, Sijin
Journal: Science Bulletin (2019)
Loss of Integrity of Doxorubicin Liposomes during Transcellular Transportation Evidenced by Fluorescence Resonance Energy Transfer Effect
Authors: Li, Dong and Zhuang, Jie and Yang, Yinqian and Wang, D and an , undefined and Yang, Jinlong and He, Haisheng and Fan, Wufa and Banerjee, Amrita and Lu, Yi and Wu, Wei and others, undefined
Journal: Colloids and Surfaces B: Biointerfaces (2018)
DOTA Functionalized Cross-Linked Small-Molecule Micelles for Theranostics Combining Magnetic Resonance Imaging and Chemotherapy
Authors: Feng, Jianyue and Luo, Qiang and Chen, Yun and Li, Bing and Luo, Kui and Lan, Jingbo and Yu, Yunlong and Zhang, Shiyong
Journal: Bioconjugate chemistry (2018)
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Authors: Hong, Hyeonjun and Huh, Man-Il and Park, Sang Min and Lee, Kyoung-pil and Kim, Hong Kyun and Kim, Dong Sung
Journal: Biofabrication (2018)
Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
The accelerated blood clearance phenomenon of PEGylated nanoemulsion upon cross administration with nanoemulsions modified with polyglycerin
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
In vivo imaging system for explants analysis–A new approach for assessment of cell transplantation effects in large animal models
Authors: Zarychta-Wisniewska, Weronika and Burdzinska, Anna and Zagozdzon, Radoslaw and Dybowski, Bartosz and Butrym, Marta and Gajewski, Zdzislaw and Paczek, Leszek
Journal: PloS one (2017): e0184588
On-Demand Drug Releasing from Dual Targeting Small Nanoparticles Triggered by High Intensity Focused Ultrasound Enhanced Glioblastoma Targeting Therapy
Authors: Luo, Zimiao and Jin, Kai and Pang, Qiang and shen, shun and Yan, Zhiqiang and Jiang, Ting and Zhu, Xiaoyan and Yu, Lei and Pang, Zhiqing and Jiang, Xinguo
Journal: ACS Applied Materials & Interfaces (2017)

References


View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483