| Basis of differentiation | Bacteria | Fungi | 
| Definition | Are single-celled microorganisms that lack a nucleus and other membrane-bound organelles | Are a group of organisms that produce spores and feed on organic matter | 
| Characteristics | Does not have a nucleus or cell organelles, and possess a cell wall made of peptidoglycan | Possesses cell organelles and nucleus, and its cell wall is made of chitin | 
| Producers/decomposers | Can be producers (photosynthesis bacteria) or decomposers (soil) | Are generally decomposers | 
| Presence of sterols | Are not present, except in mycoplasma | Are present | 
| Optimal pH | Neutral pH (6.5-7) | Slightly acidic pH (4-6) | 
| Energy source | Proteins, fats, sugars | From already existing sources from the environment | 
| Movement | Through flagella | Non-motile | 
| Reproduction | Asexually (binary fission) | Can be sexual or asexual | 
| Shape | Round (cocci),Rod (Bacillus), Spiral (Spirella), | primarily consist of thread-like structures called hyphae, though their shapes can vary. |