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AAT Bioquest

What are the differences between competitive and noncompetitive inhibition?

Posted March 9, 2023


Answer

There are a few major differences between competitive and noncompetitive inhibition in enzymes

Competitive Inhibition

  • Competitive inhibition refers to a type of enzyme inhibition in which an inhibitor molecule binds to the active sites of an enzyme. This prevents the substrate from binding to the enzyme. In this type of enzyme inhibition, inhibitors compete with the substrate for active sites.
  • Competitive inhibitors have a similar shape to that of substrate. 
  • The shape of the active site does undergo any change when a competitive inhibitor binds to the active site.
  • The competitive inhibitor and the substrate cannot be found on an enzyme at the same time.
  • The binding of competitive inhibitors with the active site is temporary and reversible.

Noncompetitive Inhibition

  • Noncompetitive inhibition refers to a type of enzyme inhibition in which an inhibitor molecule suppresses the activity of an enzyme. In this type of enzyme inhibition, the inhibitors do not compete with the substrate for active sites.
  • Noncompetitive inhibitors attach somewhere other than the active site.
  • The active site undergoes a conformational change when a noncompetitive inhibitor binds to the enzyme. 
  • The noncompetitive inhibitor and the substrate can be found on an enzyme at the same time.
  • The binding of noncompetitive inhibitors with the active site is permanent and irreversible.
Additional resources

Using Carbohydrate Interaction Assays to Reveal Novel Binding Sites in Carbohydrate Active Enzymes

Enzymes