logo
AAT Bioquest

What are the differences between Krebs cycle and glycolysis?

Posted March 30, 2023


Answer

Basis of differentiation 

Krebs cycle

Glycolysis 

Definition 

Refers to a series of chemical reactions in which pyruvate is converted to acetyl-CoA and is completely oxidized to carbon dioxide and water - also known as the citric acid cycle or tricarboxylic acid cycle (TCA cycle)

Refers to the series of chemical reaction in which a glucose molecule is degraded and broken down into two pyruvate (pyruvic acid) molecules

Order of occurrence

Is the second step of cellular respiration

Is the first step of cellular respiration

Place of occurence

Occurs in the mitochondria of eukaryotes

Occurs in the cytoplasm

Aerobic/Anaerobic Respiration

Occurs only in aerobic respiration

occurs in aerobic and anaerobic respiration

Oxygen requirement

Oxygen is used as terminal oxidant

Is not required 

Oxidative Phosphorylation

Is connected with the oxidative phosphorylation

Is not connected with the oxidative phosphorylation

Process 

Oxidizes pyruvate into carbon dioxide and water

Degrades glucose into two pyruvate molecules 

Pathway

Cyclic pathway

Linear pathway

ATP consumption 

Does not consume ATP

Consumes 2 ATP molecules

Net gain 

6 NADH molecules and 2 FADH2 molecules are produced

 

2 ATP molecules, 2 Pyruvate molecules, and 2 NADH molecules are produced

Net gain of energy 

Net energy gain is equal to 24 ATP molecules 

Net energy gain is equal to 8 ATP molecules

Carbon dioxide

Carbon dioxide is released 

No carbon dioxide is released 

End product

Is an inorganic carbon substance

Is an organic substance

Additional resources

Respiratory metabolism: glycolysis, the TCA cycle and mitochondrial electron transport

Cellular Processes

Amplite® Colorimetric Pyruvate Assay Kit

Amplite® Fluorimetric Coenzyme A Quantitation Kit *Green Fluorescence*