AAT Bioquest

Does ATP synthesis require oxygen?

Posted March 30, 2023


ATP synthesis does require oxygen. ATP formation occurs in the electron transport chain through oxidative phosphorylation. This process is catalyzed by the enzyme ATP synthase. Oxygen is required for the oxidative phosphorylation step as it synthesizes ATP molecules. Oxygen molecules act as a final hydrogen acceptor, and this produces the proton gradient that is used during ATP synthesis.    

Additional resources

ATP synthesis and storage

Cellular Processes

PhosphoWorks™ Luminometric ATP Assay Kit *DTT-Free*

Cell Meter™ Live Cell ATP Assay Kit