What are the differences between phosphorylase and phosphatase?
Posted February 10, 2023
Phosphorylase and phosphatase are two types of enzymes that catalyze biochemical reactions involving substrates with phosphate groups. There are a few significant differences between the two.
Phosphorylase
Phosphorylase catalyzes reactions that involve the transfer of phosphate groups between compounds. It plays a key role in adding a phosphate group to a phosphate acceptor.
The main substrate involved is inorganic phosphate compound.
Phosphorylase enzymes are classified into two major groups - Glycosyltransferases and Nucleotidyltransferases.
Phosphatase
Phosphatase catalyzes reactions that involve removing a phosphate group from a compound. It uses water to cleave phosphoric acid, resulting in the formation of phosphate ions and an alcohol byproduct.
Phosphoric acid is the main substrate.
Phosphoric acid can be classified into a number of different enzyme types including about 104 families.
Phosphorylase phosphatase. Comparison of active forms using peptide substrates
Amplite® Colorimetric Alkaline Phosphatase Assay Kit *Yellow Color*