Basis of differentiation | Static quenching | Dynamic quenching |
Definition | Static quenching occurs when molecules create a complex in the ground state, before excitation occurs | Dynamic quenching is the interaction of an excited state fluorophore with the quencher that concludes in deactivation of the fluorophore to the ground state |
Effect of temperature | If temperature increases, the rate of static quenching will decrease (chemical stability changes) | If temperature increases, the dynamic quenching rate will also increase |
Lifetime | The excited state lifetime is not variable | The excited state lifetime is variable |
Measurement technique | It is measured using a spectrophotometer | It is measured using a spectrofluorometer |
Fluorophore absorption spectrum | Fluorophore absorption spectrum is distorted | Fluorophore absorption spectrum remains not distorted |