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What are the differences between static quenching and dynamic quenching?
Posted January 13, 2023
Bioconjugation Chemical Reagents Förster Resonance Energy Transfer (FRET)Classic DyesPhysiological ProbesPolymerase Chain Reaction (PCR)Tide Quencher Dyes
Answer
Basis of differentiation | Static quenching | Dynamic quenching |
Definition | Static quenching occurs when molecules create a complex in the ground state, before excitation occurs | Dynamic quenching is the interaction of an excited state fluorophore with the quencher that concludes in deactivation of the fluorophore to the ground state |
Effect of temperature | If temperature increases, the rate of static quenching will decrease (chemical stability changes) | If temperature increases, the dynamic quenching rate will also increase |
Lifetime | The excited state lifetime is not variable | The excited state lifetime is variable |
Measurement technique | It is measured using a spectrophotometer | It is measured using a spectrofluorometer |
Fluorophore absorption spectrum | Fluorophore absorption spectrum is distorted | Fluorophore absorption spectrum remains not distorted |
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