Basis of differentiation | Substrate | Product |
Definition | Substrate refers to the compound that the enzyme acts on and modifies in a reaction. It is the starting material in a reaction. | Product refers to the compound that is obtained when the reaction is complete. It is the end result of a reaction. |
Concentration at the start of the reaction | There is a high concentration of substrates at the start of the reaction. | There are no products formed yet at the start of the reaction. |
Concentration as the reaction progresses | The concentration of the substrate decreases as the reaction progresses. | The concentration of the product increases as the reaction progresses. |
Concentration at the end of the reaction | There may be no or very low concentration of substrates at the end of the reaction. | There is a high concentration of products at the end of the reaction. |
Number of substrates/products in a reaction | There may be one or more substrates involved in a reaction. | There may be one or more products formed at the end of a reaction. |
Example | The enzyme lactase acts on the substrate lactose, which is a sugar found in milk. | The reaction between the enzyme (lactase) and the substrate (lactose) results two products – glucose and galactose. |