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What are the disadvantages of indirect detection methods in immunohistochemistry (IHC)?
Posted May 2, 2024
Antibodies and Proteomics BioconjugationAntibody and Protein LabelingImmunohistochemistry (IHC)
Answer
  • The binding between the secondary antibody and other proteins present on the blot can give rise to more non-specific signals. 
  • Additional incubation, blocking, and wash steps add time to the process.  
Additional resources
Multiplex Spatial Protein Detection by Combining Immunofluorescence with Immunohistochemistry Immunohistochemistry (IHC) Stayright™ Purple HRP Staining Kit

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