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AAT Bioquest

What are the limitations of a two-way ANOVA?

Posted May 19, 2022


Answer

These are the limitations found in a two-way ANOVA: 

  • It becomes difficult to maintain homogeneity of the blocks if the number of treatments is large enough. 
  • The technique can be challenging and time-consuming. 
  • In order to get accurate results, a missing value cannot be ignored. Certain adjustments must be made in the analysis after replacing the missing value with some function of the existing values. 
  • Analysis becomes increasingly more complex and interpretation more difficult when the number of the factors are increased in a study. 
Additional resources

Generalized F test and generalized deviance test in two-way ANOVA models for randomized trials

ANOVA Calculator