The lock-and-key model proposes that the enzyme’s active site complements the shape of the substrate molecule so both fit together like a lock and key. If their shapes are not complementary, they are unable to bind as no structural changes occur.
Limitations of the lock and key model include:
Assumes that enzymes have a rigid structure and do not change shape on binding with an appropriate substrate
Does not account for the intricate chemistry of catalytic reactions
Does not offer an explanation of how the enzyme-substrate complex is stabilized in the transition state
Does not offer an explanation for binding multiple substrates to the enzyme
Does not take proton transfer mechanisms into consideration