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What are the limitations of SDS-PAGE electrophoresis?
Posted July 17, 2024

Answer

Limitations of SDS PAGE

  • Polyacrylamide gel preparation is time consuming
  • Gel preparation requires extreme care to prevent leaking
  • Gels cannot be reused in an experiment
  • Not effective for identifying DNA or RNA structure of a strain
  • Not effective for determining protein purity 
  • The process uses toxic monomers