SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe

In SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), SDS Running Buffer is used as the electrophoresis buffer during stacking and resolution.

To prepare

L of SDS-PAGE SDS Running Buffer (10x):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Tris base (mw: 121.14 g / mol)	#	#
Glycine (mw: 75.07 g / mol)	#	#
SDS (mw: 288.38 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Tris base to the solution
Add # of Glycine to the solution
Add # of SDS to the solution
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe." AAT Bioquest, Inc., 24 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sds-page-10x-sds-running-buffer.
APA	AAT Bioquest, Inc. (2025, November 24). Quest Calculate™ SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sds-page-10x-sds-running-buffer.
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