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SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe

In SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), SDS Running Buffer is used as the electrophoresis buffer during stacking and resolution.

To prepare  L of SDS-PAGE SDS Running Buffer (10x):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Tris base (mw: 121.14 g/mol)30.3 g0.2501 M
Glycine (mw: 75.07 g/mol)144.4 g1.924 M
SDS (mw: 288.38 g/mol)10 g0.03467 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 30.3 g of Tris base to the solution.
  3. Add 144.4 g of Glycine to the solution.
  4. Add 10 g of SDS to the solution.
  5. Add distilled water until the volume is 1 L.

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This online tool may be cited as follows


"Quest Calculate™ SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe." AAT Bioquest, Inc.21 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/sds-page-10x-sds-running-buffer.


AAT Bioquest, Inc. (2024June 21). Quest Calculate™ SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sds-page-10x-sds-running-buffer.