Products
Technologies
Applications
Services
Resources
Selection Guides
About
SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe
In SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis), SDS Running Buffer is used as the electrophoresis buffer during stacking and resolution.
To prepareL of SDS-PAGE SDS Running Buffer (10x):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Tris base (mw: 121.14 g / mol) | # | # |
| Glycine (mw: 75.07 g / mol) | # | # |
| SDS (mw: 288.38 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Tris base to the solution
- Add # of Glycine to the solution
- Add # of SDS to the solution
- Add distilled water until the volume is 1 L.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe." AAT Bioquest, Inc., 9 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sds-page-10x-sds-running-buffer. | |
APA | AAT Bioquest, Inc. (2025, October 9). Quest Calculate™ SDS-PAGE SDS Running Buffer (10x) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sds-page-10x-sds-running-buffer. | |
| BibTeX | EndNote | RefMan |