Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe

Sodium Borate Buffer is a buffer that is used to maintain the pH within a relatively narrow range. It is isotonic and has a strong bactericidal effect, and can be used to dilute substances or in coating procedures.

To prepare

L of Sodium Borate Buffer (1 M, pH 8.5):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Boric Acid (H3BO3) (mw: 61.83 g / mol)	#	#
Sodium Hydroxide (mw: 40 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of Boric Acid (H3BO3) to the solution
Add # of Sodium Hydroxide to the solution
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe." AAT Bioquest, Inc., 25 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-borate-buffer-ph-8-5.
APA	AAT Bioquest, Inc. (2025, October 25). Quest Calculate™ Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-borate-buffer-ph-8-5.
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