Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe

Sodium Borate Buffer is a buffer that is used to maintain the pH within a relatively narrow range. It is isotonic and has a strong bactericidal effect, and can be used to dilute substances or in coating procedures.

To prepare L of Sodium Borate Buffer (1 M, pH 8.5):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Boric Acid (H3BO3) (mw: 61.83 g/mol)	61.83 g	1 M
Sodium Hydroxide (mw: 40.00 g/mol)	10 g	0.25 M

Prepare 800 mL of dH2O in a suitable container.
Add 61.83 g of Boric Acid (H3BO3) to the solution.
Add 10 g of Sodium Hydroxide to the solution.
Add dH2O until the volume is 1 L.

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Physiological Buffer

pH Buffering

Sample Preparation

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PIPES Buffer (1 M, 6.8 pH)
Potassium Phosphate Buffer (1 M, pH 6.5)
Potassium Phosphate (pH 5.8 to 8.0)
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Sodium Borate Buffer (1 M, pH 8.5)
Sodium Phosphate Solution
SSC buffer (20X) (3 M, pH 7)
TAE (1 M, pH 8.6)
TB Buffer
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TES Buffer (1 M, 7.5 pH)
Tricine (1 M, pH 8.05)
Tris Buffer (1 M, pH 7.2)
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References

This online tool may be cited as follows

MLA	"Quest Calculate™ Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe." AAT Bioquest, Inc., 20 Apr. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-borate-buffer-ph-8-5.
APA	AAT Bioquest, Inc. (2024, April 20). Quest Calculate™ Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-borate-buffer-ph-8-5.
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Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe

References

This online tool may be cited as follows

MLA

APA