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Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe

Sodium Borate Buffer is a buffer that is used to maintain the pH within a relatively narrow range. It is isotonic and has a strong bactericidal effect, and can be used to dilute substances or in coating procedures.

To prepare  L of Sodium Borate Buffer (1 M, pH 8.5):
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Table 1. Required components
Boric Acid (H3BO3) (mw: 61.83 g/mol)61.83 g1 M
Sodium Hydroxide (mw: 40.00 g/mol)10 g0.25 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 61.83 g of Boric Acid (H3BO3) to the solution.
  3. Add 10 g of Sodium Hydroxide to the solution.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe." AAT Bioquest, Inc.25 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-borate-buffer-ph-8-5.


AAT Bioquest, Inc. (2024May 25). Quest Calculate™ Sodium Borate Buffer (1 M, pH 8.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-borate-buffer-ph-8-5.