Sodium Acetate (3 M, pH 5.2) Preparation and Recipe
Sodium Acetate (3 M) is commonly used during DNA and RNA extraction. Sodium acetate, which is a salt, helps precipitate nucleic acids.
To prepare L of Sodium Acetate (3 M, pH 5.2):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Sodium Acetate (mw: 82.03 g/mol) | 246.1 g | 3 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 246.1 g of Sodium Acetate to the solution.
- Adjust the pH to 5.2 with glacial acetic acid. Allow the solution to cool overnight. Adjust the pH once more to 5.2 with glacial acetic acid.
- Add distilled water until the volume is 1 L.
- Filter-sterilize the solution.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Sodium Acetate (3 M, pH 5.2) Preparation and Recipe." AAT Bioquest, Inc., 8 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-acetate-3-m-ph-5-2. | |
APA | AAT Bioquest, Inc. (2024, October 8). Quest Calculate™ Sodium Acetate (3 M, pH 5.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-acetate-3-m-ph-5-2. | |
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