AAT Bioquest

Sodium Acetate (3 M, pH 5.2) Preparation and Recipe

Sodium Acetate (3 M) is commonly used during DNA and RNA extraction. Sodium acetate, which is a salt, helps precipitate nucleic acids.

To prepare  L of Sodium Acetate (3 M, pH 5.2):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium Acetate (mw: 82.03 g/mol)246.1 g3 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 246.1 g of Sodium Acetate to the solution.
  3. Adjust the pH to 5.2 with glacial acetic acid. Allow the solution to cool overnight. Adjust the pH once more to 5.2 with glacial acetic acid.
  4. Add distilled water until the volume is 1 L.
  5. Filter-sterilize the solution.

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Physiological Buffer
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This online tool may be cited as follows


"Quest Calculate™ Sodium Acetate (3 M, pH 5.2) Preparation and Recipe." AAT Bioquest, Inc.20 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-acetate-3-m-ph-5-2.


AAT Bioquest, Inc. (2024June 20). Quest Calculate™ Sodium Acetate (3 M, pH 5.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-acetate-3-m-ph-5-2.

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