Sodium Acetate (3 M, pH 5.2) Preparation and Recipe

Sodium Acetate (3 M) is commonly used during DNA and RNA extraction. Sodium acetate, which is a salt, helps precipitate nucleic acids.

To prepare L of Sodium Acetate (3 M, pH 5.2):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium Acetate (mw: 82.03 g/mol)	246.1 g	3 M

Prepare 800 mL of distilled water in a suitable container.
Add 246.1 g of Sodium Acetate to the solution.
Adjust the pH to 5.2 with glacial acetic acid. Allow the solution to cool overnight. Adjust the pH once more to 5.2 with glacial acetic acid.
Add distilled water until the volume is 1 L.
Filter-sterilize the solution.

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Physiological Buffer

pH Buffering

Sample Preparation

BioAssays

Misc

Cell/Culture/Growth Media

Gel Electrophoresis

Name ▲ ▼
Extracellular Saline (1x, pH 7.3)
HBSS (Hank's Balanced Salt Solution)
HEPES Buffer (1 M, 7.5 pH)
HHBS (Hank's Buffer with Hepes)
PBS (Phosphate Buffered Saline) (1X, pH 7.4)
PBS with Ca2+and Mg2+ (D-PBS)
Tyrode's Solution, Acidic

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ACES Buffer (0.1 M, 6.7 pH)
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Acetate Buffer (pH 3.6 to 5.6)
ADA Buffer (0.5 M, 6.6 pH)
Ammonium Acetate
Ammonium Bicarbonate (50 mM, pH 7.8)
Ammonium Sulfate, Saturated
BES-Buffered Saline (2X) (0.05 M, pH 6.95)
Bicine (1 M, pH 8.26)
Bis-Tris Buffer (1 M, 6.5 pH)
Borate Buffer
CAPS (0.5 M, pH 10.4)
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)
CHES (0.5 M, pH 9.5)
Citrate Buffer (0.1 M, pH 6.0)
Citrate Buffer (pH 3.0 to 6.2)
Citrate-Phosphate Buffer (0.15 M, pH 5.0)
Citrate-Phosphate Buffer (110 mM, pH 5.6)
Citrate-Phosphate Buffer (40 mM, pH 5.6)
Diethanolamine (1 M, pH 9.8)
EBSS (magnesium, calcium, phenol red) (pH 7.0)
Glycine (0.1 M, pH 2.2)
Glycine-HCl Buffer (0.1 M, pH 3.0)
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)
HEPPSO (1 M, pH 7.85)
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)
Imidazole-HCl Buffer (0.05 M, pH 7.0)
Maleic Acid (1 M, pH 7.5)
MES (0.5 M, pH 6)
MOPS Buffer (10X) (0.2 M, pH 7)
MOPSO Buffer (0.5 M, 6.9 pH)
Phosphate Buffer (pH 5.8 to 7.4)
PIPES Buffer (1 M, 6.8 pH)
Potassium Phosphate Buffer (1 M, pH 6.5)
Potassium Phosphate (pH 5.8 to 8.0)
Sodium Acetate (3 M, pH 5.2)
Sodium Borate Buffer (1 M, pH 8.5)
Sodium Phosphate Solution
SSC buffer (20X) (3 M, pH 7)
TAE (1 M, pH 8.6)
TB Buffer
TBS (1 M, pH 7.4)
TES Buffer (1 M, 7.5 pH)
Tricine (1 M, pH 8.05)
Tris Buffer (1 M, pH 7.2)
Tris-Buffered Saline (TBS, 0.1 M)
Tris-citrate Buffer (pH 8.0)
Trizma® Buffer (pH 7.0 to 9.2)

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DEAE-dextran
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Elution Buffer
Gey's Balanced Salt Solution
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Paraformaldehyde Solution (8%)
TE Buffer 10X
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Anesthetic (MS222) Solution
Bradford Stock Solution
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Chloroquine
Cresol Red Solution
Ethidium Bromide Solution
Immunofluorescence Quenching Solution
Mayer's Hemalum
Nuclear Fast Red Solution
Pyronin Y (PY) Solution
SSC (Northern Blot, 20x)
SSPE (Blot, 20x, pH 7.4)

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Dithiothreitol (DTT) (1 M)
Sodium Iodide/Sodium Sulfite Solution

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Bath Solution (Ca2+/Mg2+ Free)
Calcium Chloride Stock Solution
Carbenicillin Solution
Chelation Medium
Digestion Medium
Embryonic Medium
IPTG Solution
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Tris/EACA Buffer
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References

This online tool may be cited as follows

MLA	"Quest Calculate™ Sodium Acetate (3 M, pH 5.2) Preparation and Recipe." AAT Bioquest, Inc., 24 Apr. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-acetate-3-m-ph-5-2.
APA	AAT Bioquest, Inc. (2024, April 24). Quest Calculate™ Sodium Acetate (3 M, pH 5.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-acetate-3-m-ph-5-2.
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This online tool has been cited in 3 publications, including

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Journal: Industrial \& Engineering Chemistry Research (2023)

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Sodium Acetate (3 M, pH 5.2) Preparation and Recipe

References

This online tool may be cited as follows

MLA

APA

This online tool has been cited in 3 publications, including