Sodium Acetate (3 M, pH 5.2) Preparation and Recipe

Sodium Acetate (3 M) is commonly used during DNA and RNA extraction. Sodium acetate, which is a salt, helps precipitate nucleic acids.

To prepare

L of Sodium Acetate (3 M, pH 5.2):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium Acetate (mw: 82.03 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium Acetate to the solution
Adjust the pH to 5.2 with glacial acetic acid. Allow the solution to cool overnight. Adjust the pH once more to 5.2 with glacial acetic acid.
Add distilled water until the volume is 1 L.
Filter-sterilize the solution.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Sodium Acetate (3 M, pH 5.2) Preparation and Recipe." AAT Bioquest, Inc., 10 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-acetate-3-m-ph-5-2.
APA	AAT Bioquest, Inc. (2025, October 10). Quest Calculate™ Sodium Acetate (3 M, pH 5.2) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/sodium-acetate-3-m-ph-5-2.
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This online resource has been cited in 3 publications, including

Authors:

Li, Beibei and Yao, Tuo and Tao, Tiantian and Han, Dandan and Yang, Huaiyu and Gao, Zhenguo and Gong, Junbo

Journal:

Industrial \& Engineering Chemistry Research (2023)

Authors:

Olubode, Aderemi

Authors:

Lum Bongam, Alvine