AAT Bioquest

CHES (0.5 M, pH 9.5) Preparation and Recipe

CHES is a dipolar ion (Zwitterionic) used at relatively high pH levels up to 10. It is employed in several biological research fields including enzymology, where it is useful for studying reactions above physiological pH range. Commonly used for electrophoresis, it has low reactivity with most metal ions.

To prepare  L of CHES (0.5 M, pH 9.5):
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Table 1. Required components
CHES (mw: 207.29 g/mol)103.65 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 103.65 g of CHES to the solution.
  3. Adjust solution to desired pH with 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ CHES (0.5 M, pH 9.5) Preparation and Recipe." AAT Bioquest, Inc.20 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/ches-ph-9-5.


AAT Bioquest, Inc. (2024April 20). Quest Calculate™ CHES (0.5 M, pH 9.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ches-ph-9-5.