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CHES (0.5 M, pH 9.5) Preparation and Recipe

CHES is a dipolar ion (Zwitterionic) used at relatively high pH levels up to 10. It is employed in several biological research fields including enzymology, where it is useful for studying reactions above physiological pH range. Commonly used for electrophoresis, it has low reactivity with most metal ions.

To prepare  L of CHES (0.5 M, pH 9.5):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
CHES (mw: 207.29 g/mol)103.65 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 103.65 g of CHES to the solution.
  3. Adjust solution to desired pH with 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ CHES (0.5 M, pH 9.5) Preparation and Recipe." AAT Bioquest, Inc.7 Dec2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/ches-ph-9-5.

APA

AAT Bioquest, Inc. (2022December 7). Quest Calculate™ CHES (0.5 M, pH 9.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ches-ph-9-5.