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CHES (0.5 M, pH 9.5) Preparation and Recipe
CHES is a dipolar ion (Zwitterionic) used at relatively high pH levels up to 10. It is employed in several biological research fields including enzymology, where it is useful for studying reactions above physiological pH range. Commonly used for electrophoresis, it has low reactivity with most metal ions.
To prepare
L of CHES (0.5 M, pH 9.5):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
CHES (mw: 207.29 g / mol)##
  • Prepare 800 mL of dH2O in a suitable container.
  • Add # of CHES to the solution
  • Adjust solution to desired pH with 10N NaOH.
  • Add dH2O until the volume is 1 L.


References
This online resource may be cited as follows
MLA
"Quest CalculateCHES (0.5 M, pH 9.5) Preparation and Recipe." AAT Bioquest, Inc.13 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/ches-ph-9-5.
APA
AAT Bioquest, Inc. (2025October 13). Quest CalculateCHES (0.5 M, pH 9.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ches-ph-9-5.
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