CHES (0.5 M, pH 9.5) Preparation and Recipe

CHES is a dipolar ion (Zwitterionic) used at relatively high pH levels up to 10. It is employed in several biological research fields including enzymology, where it is useful for studying reactions above physiological pH range. Commonly used for electrophoresis, it has low reactivity with most metal ions.

To prepare

L of CHES (0.5 M, pH 9.5):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
CHES (mw: 207.29 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of CHES to the solution
Adjust solution to desired pH with 10N NaOH.
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ CHES (0.5 M, pH 9.5) Preparation and Recipe." AAT Bioquest, Inc., 31 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ches-ph-9-5.
APA	AAT Bioquest, Inc. (2025, October 31). Quest Calculate™ CHES (0.5 M, pH 9.5) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ches-ph-9-5.
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