AAT Bioquest

TE Buffer 10X Preparation and Recipe

TE buffer is used as a protective measure against DNA and RNA degredation, storing the two molecules and maintaining proper pH levels.

To prepare  L of TE Buffer 10X:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Tris-Cl (desired pH) (mw: 157.594 g/mol)15.759 g0.1 M
EDTA (pH 8) (mw: 292.24 g/mol)2.92 g0.01 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 15.759 g of Tris-Cl (desired pH) to the solution.
  3. Add 2.92 g of EDTA (pH 8) to the solution.
  4. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ TE Buffer 10X Preparation and Recipe." AAT Bioquest, Inc.18 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/te-buffer-10x.


AAT Bioquest, Inc. (2024April 18). Quest Calculate™ TE Buffer 10X Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/te-buffer-10x.