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Modified Barth's Saline (MBS) Preparation and Recipe

Modified Barth's Saline (MBS) is commonly used as culture media for Xenopus oocytes

To prepare  L of Modified Barth's Saline (MBS):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Hepes (mw: 238.30 g/mol)11.92 g0.05002 M
Sodium chloride (mw: 58.44 g/mol)51.43 g0.88 M
Potassium Chloride (mw: 74.55 g/mol)0.75 g0.01006 M
Sodium bicarbonate (mw: 84.01 g/mol)2.1 g0.02499 M
Magnesium Sulfate Heptahydrate (mw: 246.47 g/mol)2.46 g0.00998 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 11.92 g of Hepes to the solution.
  3. Add 51.43 g of Sodium chloride to the solution.
  4. Add 0.75 g of Potassium Chloride to the solution.
  5. Add 2.1 g of Sodium bicarbonate to the solution.
  6. Add 2.46 g of Magnesium Sulfate Heptahydrate to the solution.
  7. Adjust the pH of 10x MBS salt solution to 7.8 with 10 M NaOH, and sterilize by autoclaving. Store indefinitely at room temperature.
  8. Add distilled water until the volume is 1 L.
  9. To prepare 1x MBS, combine 100 mL of 10x MBS salt solution and 7 mL of 0.1 M CaCl2 with 893 mL of autoclaved deionized H2O. Add antibiotics. Store for up to 1-2 mo at 16°C-18°C (the same temperature used for oocyte incubation). Check before use.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Modified Barth's Saline (MBS) Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/modified-barth-s-saline-mbs-for-oocytes.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ Modified Barth's Saline (MBS) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/modified-barth-s-saline-mbs-for-oocytes.