HBSS (Hank's Balanced Salt Solution) recipe and preparation

Maintains physiological pH and osmotic balance for cells.

To prepare L of HBSS (Hank's Balanced Salt Solution):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
NaCl (mw: 58.4 g/mol)8 g0.14 M
KCl (mw: 74.551 g/mol)400 mg0.005 M
CaCl2 (mw: 110.98 g/mol)140 mg0.001 M
MgSO4-7H2O (mw: 246.475 g/mol)100 mg0.0004 M
MgCl2-6H2O (mw: 203.303 g/mol)100 mg0.0005 M
Na2HPO4-2H2O (mw: 177.99 g/mol)60 mg0.0003 M
KH2PO4 (mw: 136.086 g/mol)60 mg0.0004 M
Glucose (mw: 180.156 g/mol)1 g0.006 M
NaHCO3 (mw: 84.007 g/mol)350 mg0.004 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 8 g of NaCl to the solution.
  3. Add 400 mg of KCl to the solution.
  4. Add 140 mg of CaCl2 to the solution.
  5. Add 100 mg of MgSO4-7H2O to the solution.
  6. Add 100 mg of MgCl2-6H2O to the solution.
  7. Add 60 mg of Na2HPO4-2H2O to the solution.
  8. Add 60 mg of KH2PO4 to the solution.
  9. Add 1 g of Glucose to the solution.
  10. Add 350 mg of NaHCO3 to the solution.
  11. Add distilled water until volume is 1 L.


Min pH

Max pH


Acetate Buffer (0.1 M, pH 5.0)3.75.6Common Physiological
BES-Buffered Saline (2X) (0.05 M, pH 6.95)6.47.8Common Physiological
Bicine (1 M, pH 8.26)7.69.0Common Physiological
CAPS (0.5 M, pH 10.4)9.711.1Common Physiological
CHES (0.5 M, pH 9.5)8.610.0Common Physiological
Citrate Buffer (0.1 M, pH 6.0)3.06.2Common Physiological
Citrate-Phosphate Buffer (0.15 M, pH 5.0)2.67.6Common Physiological
Diethanolamine (1 M, pH 9.8)7.89.9Common Physiological
EBSS (magnesium, calcium, phenol red) (pH 7.0)7.07.4Common Physiological
Glycine-HCl Buffer (0.1 M, pH 3.0)2.23.6Common Physiological
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)8.610.6Common Physiological
HBSS (Hank's Balanced Salt Solution)7.27.6Common Physiological
HEPPSO (1 M, pH 7.85)7.18.5Common Physiological
HHBS (Hank's Buffer with Hepes)7.27.4Common Physiological
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)1.02.2Common Physiological
Imidazole-HCl Buffer (0.05 M, pH 7.0)6.27.8Common Physiological
Maleic Acid (1 M, pH 7.5)7.08.0Common Physiological
MES (0.5 M, pH 6)5.56.7Common Physiological
MOPS Buffer (10X) (0.2 M, pH 7)6.57.9Common Physiological
PBS (Phosphate Buffered Saline) (1X, pH 7.4)7.37.5Common Physiological
Sodium Borate Buffer (1 M, pH 8.5)8.010.0Common Physiological
TAE (1 M, pH 8.6)8.08.5Common Physiological
TAE Buffer (50X) (0.04 M, pH 8.5)8.08.5Common Physiological
TBS (1 M, pH 7.4)7.27.5Common Physiological
TE Buffer 10X7.58.5Common Physiological
Tricine (1 M, pH 8.05)7.48.8Common Physiological
Tris Buffer (1 M, pH 7.0)7.07.5Common Physiological
Acetate Buffer (pH 3.6 to 5.6)3.75.6Variable pH
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)9.210.8Variable pH
Citrate Buffer (pH 3.0 to 6.2)3.06.2Variable pH
Phosphate Buffer (pH 5.8 to 7.4)5.88.0Variable pH
Potassium Phosphate (pH 5.8 to 8.0)5.88.0Variable pH
Trizma Buffer (pH 7.2 to 9.0)7.29.0Variable pH