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HBSS (Hank's Balanced Salt Solution) Preparation and Recipe
Maintains physiological pH and osmotic balance for cells.
To prepare
L of HBSS (Hank's Balanced Salt Solution):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Sodium chloride (mw: 58.44 g / mol)##
Potassium Chloride (mw: 74.55 g / mol)##
Calcium Chloride (mw: 110.98 g / mol)##
Magesium Sulfate Heptahydrate (mw: 246.47 g / mol)##
Magnesium Chloride Hexahydrate (mw: 203.3 g / mol)##
Sodium Phosphate Dibasic Dihydrate (mw: 177.99 g / mol)##
Potassium Phosphate Monobasic (mw: 136.09 g / mol)##
D-Glucose (Dextrose) (mw: 180.16 g / mol)##
Sodium bicarbonate (mw: 84.01 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Sodium chloride to the solution
  • Add # of Potassium Chloride to the solution
  • Add # of Calcium Chloride to the solution
  • Add # of Magesium Sulfate Heptahydrate to the solution
  • Add # of Magnesium Chloride Hexahydrate to the solution
  • Add # of Sodium Phosphate Dibasic Dihydrate to the solution
  • Add # of Potassium Phosphate Monobasic to the solution
  • Add # of D-Glucose (Dextrose) to the solution
  • Add # of Sodium bicarbonate to the solution
  • Add distilled water until the volume is 1 L.


References
This online resource may be cited as follows
MLA
"Quest CalculateHBSS (Hank's Balanced Salt Solution) Preparation and Recipe." AAT Bioquest, Inc.10 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/hbss-hanks-balanced-salt-solution.
APA
AAT Bioquest, Inc. (2025October 10). Quest CalculateHBSS (Hank's Balanced Salt Solution) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hbss-hanks-balanced-salt-solution.
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