HBSS (Hank's Balanced Salt Solution) Preparation and Recipe

Maintains physiological pH and osmotic balance for cells.

To prepare

L of HBSS (Hank's Balanced Salt Solution):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g / mol)	#	#
Potassium Chloride (mw: 74.55 g / mol)	#	#
Calcium Chloride (mw: 110.98 g / mol)	#	#
Magesium Sulfate Heptahydrate (mw: 246.47 g / mol)	#	#
Magnesium Chloride Hexahydrate (mw: 203.3 g / mol)	#	#
Sodium Phosphate Dibasic Dihydrate (mw: 177.99 g / mol)	#	#
Potassium Phosphate Monobasic (mw: 136.09 g / mol)	#	#
D-Glucose (Dextrose) (mw: 180.16 g / mol)	#	#
Sodium bicarbonate (mw: 84.01 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium chloride to the solution
Add # of Potassium Chloride to the solution
Add # of Calcium Chloride to the solution
Add # of Magesium Sulfate Heptahydrate to the solution
Add # of Magnesium Chloride Hexahydrate to the solution
Add # of Sodium Phosphate Dibasic Dihydrate to the solution
Add # of Potassium Phosphate Monobasic to the solution
Add # of D-Glucose (Dextrose) to the solution
Add # of Sodium bicarbonate to the solution
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ HBSS (Hank's Balanced Salt Solution) Preparation and Recipe." AAT Bioquest, Inc., 7 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/hbss-hanks-balanced-salt-solution.
APA	AAT Bioquest, Inc. (2025, November 7). Quest Calculate™ HBSS (Hank's Balanced Salt Solution) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hbss-hanks-balanced-salt-solution.
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