AAT Bioquest

HBSS (Hank's Balanced Salt Solution) Preparation and Recipe

Maintains physiological pH and osmotic balance for cells.

To prepare  L of HBSS (Hank's Balanced Salt Solution):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)8 g0.14 M
Potassium Chloride (mw: 74.55 g/mol)0.4 g0.005 M
Calcium Chloride (mw: 110.98 g/mol)0.14 g0.001 M
Magesium Sulfate Heptahydrate (mw: 246.47 g/mol)0.1 g0.0004 M
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol)0.1 g0.0005 M
Sodium Phosphate Dibasic Dihydrate (mw: 177.99 g/mol)0.06 g0.0003 M
Potassium Phosphate Monobasic (mw: 136.09 g/mol)0.06 g0.0004 M
D-Glucose (Dextrose) (mw: 180.16 g/mol)1 g0.006 M
Sodium bicarbonate (mw: 84.01 g/mol)0.35 g0.004 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 8 g of Sodium chloride to the solution.
  3. Add 0.4 g of Potassium Chloride to the solution.
  4. Add 0.14 g of Calcium Chloride to the solution.
  5. Add 0.1 g of Magesium Sulfate Heptahydrate to the solution.
  6. Add 0.1 g of Magnesium Chloride Hexahydrate to the solution.
  7. Add 0.06 g of Sodium Phosphate Dibasic Dihydrate to the solution.
  8. Add 0.06 g of Potassium Phosphate Monobasic to the solution.
  9. Add 1 g of D-Glucose (Dextrose) to the solution.
  10. Add 0.35 g of Sodium bicarbonate to the solution.
  11. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ HBSS (Hank's Balanced Salt Solution) Preparation and Recipe." AAT Bioquest, Inc.30 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/hbss-hanks-balanced-salt-solution.


AAT Bioquest, Inc. (2024May 30). Quest Calculate™ HBSS (Hank's Balanced Salt Solution) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hbss-hanks-balanced-salt-solution.

This online tool has been cited in 2 publications, including

Effect of hydroxyapatite: zirconia volume fraction ratio on mechanical and corrosive properties of Ti-matrix composite scaffolds
Journal: Transactions of Nonferrous Metals Society of China (2022): 882--894
Mitigating the detrimental effects of galvanic corrosion by nanoscale composite architecture design
Journal: npj Materials Degradation (2022): 1--9