HBSS (Hank's Balanced Salt Solution) Preparation and Recipe
Maintains physiological pH and osmotic balance for cells.
To prepare L of HBSS (Hank's Balanced Salt Solution):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
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Component | Amount | Concentration |
Sodium chloride (mw: 58.44 g/mol) | 8 g | 0.14 M |
Potassium Chloride (mw: 74.55 g/mol) | 0.4 g | 0.005 M |
Calcium Chloride (mw: 110.98 g/mol) | 0.14 g | 0.001 M |
Magesium Sulfate Heptahydrate (mw: 246.47 g/mol) | 0.1 g | 0.0004 M |
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol) | 0.1 g | 0.0005 M |
Sodium Phosphate Dibasic Dihydrate (mw: 177.99 g/mol) | 0.06 g | 0.0003 M |
Potassium Phosphate Monobasic (mw: 136.09 g/mol) | 0.06 g | 0.0004 M |
D-Glucose (Dextrose) (mw: 180.16 g/mol) | 1 g | 0.006 M |
Sodium bicarbonate (mw: 84.01 g/mol) | 0.35 g | 0.004 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 8 g of Sodium chloride to the solution.
- Add 0.4 g of Potassium Chloride to the solution.
- Add 0.14 g of Calcium Chloride to the solution.
- Add 0.1 g of Magesium Sulfate Heptahydrate to the solution.
- Add 0.1 g of Magnesium Chloride Hexahydrate to the solution.
- Add 0.06 g of Sodium Phosphate Dibasic Dihydrate to the solution.
- Add 0.06 g of Potassium Phosphate Monobasic to the solution.
- Add 1 g of D-Glucose (Dextrose) to the solution.
- Add 0.35 g of Sodium bicarbonate to the solution.
- Add distilled water until the volume is 1 L.
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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ HBSS (Hank's Balanced Salt Solution) Preparation and Recipe." AAT Bioquest, Inc., 9 Dec. 2023, https://www.aatbio.com/resources/buffer-preparations-and-recipes/hbss-hanks-balanced-salt-solution. | |
APA | AAT Bioquest, Inc. (2023, December 9). Quest Calculate™ HBSS (Hank's Balanced Salt Solution) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hbss-hanks-balanced-salt-solution. | |
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This online tool has been cited in 2 publications, including
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Journal: Transactions of Nonferrous Metals Society of China (2022): 882--894
Authors:
Journal: Transactions of Nonferrous Metals Society of China (2022): 882--894
Mitigating the detrimental effects of galvanic corrosion by nanoscale composite architecture design
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Journal: npj Materials Degradation (2022): 1--9
Authors:
Journal: npj Materials Degradation (2022): 1--9