AAT Bioquest

Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix Preparation and Recipe

To prepare  L of Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Penicillin G (mw: 356.37 g/mol)10 g0.02806 M
Streptomycin (mw: 728.69 g/mol)5 g0.006861 M
Chloramphenicol (mw: 323.13 g/mol)1 g0.003094 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 10 g of Penicillin G to the solution.
  3. Add 5 g of Streptomycin to the solution.
  4. Add 1 g of Chloramphenicol to the solution.
  5. Add distilled water until the volume is 1 L.
  6. Filter through a 0.22-µm membrane and store in 5-mL aliquots at ~20°C. For use, add 5 mL of the antibiotic mix to 1 L of Provasoli-enriched seawater (PES).

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This online tool may be cited as follows


"Quest Calculate™ Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix Preparation and Recipe." AAT Bioquest, Inc.20 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/antibiotic-mix.


AAT Bioquest, Inc. (2024June 20). Quest Calculate™ Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/antibiotic-mix.