Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix Preparation and Recipe
To prepare L of Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix:
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Table 1. Required components
Component | Amount | Concentration |
Penicillin G (mw: 356.37 g/mol) | 10 g | 0.02806 M |
Streptomycin (mw: 728.69 g/mol) | 5 g | 0.006861 M |
Chloramphenicol (mw: 323.13 g/mol) | 1 g | 0.003094 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 10 g of Penicillin G to the solution.
- Add 5 g of Streptomycin to the solution.
- Add 1 g of Chloramphenicol to the solution.
- Add distilled water until the volume is 1 L.
- Filter through a 0.22-µm membrane and store in 5-mL aliquots at ~20°C. For use, add 5 mL of the antibiotic mix to 1 L of Provasoli-enriched seawater (PES).
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix Preparation and Recipe." AAT Bioquest, Inc., 5 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/antibiotic-mix. | |
APA | AAT Bioquest, Inc. (2024, October 5). Quest Calculate™ Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/antibiotic-mix. | |
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