AAT Bioquest

Mayer's Hemalum Preparation and Recipe

To prepare  L of Mayer's Hemalum:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
aluminum potassium sulfate (KAl[SO4]2•12H2O) (mw: 474.39 g/mol)50 g0.1053 M
hematoxylin (C.I. 75290) (mw: 302.28 g/mol)1 g0.003308 M
sodium iodate (NaIO3) (mw: 197.89 g/mol)0.1 g0.0005053 M
citric acid (monohydrate) (mw: 210.14 g/mol)1 g0.004758 M
chloral hydrate (mw: 165.40 g/mol)50 g0.3022 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 50 g of aluminum potassium sulfate (KAl[SO4]2•12H2O) to the solution.
  3. Add 1 g of hematoxylin (C.I. 75290) to the solution.
  4. Add 0.1 g of sodium iodate (NaIO3) to the solution.
  5. Add 1 g of citric acid (monohydrate) to the solution.
  6. Add 50 g of chloral hydrate to the solution.
  7. A magnetic stirrer will hasten dissolving of the alum, hematoxylin, and hematein formed by iodate oxidation.
  8. Add distilled water until the volume is 1 L.
  9. Mayer's hemalum often keeps for 1 yr but some batches lose their potency after only a few months. The solution may be reused many times.

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This online tool may be cited as follows


"Quest Calculate™ Mayer's Hemalum Preparation and Recipe." AAT Bioquest, Inc.20 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/mayer-s-hemalum.


AAT Bioquest, Inc. (2024June 20). Quest Calculate™ Mayer's Hemalum Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mayer-s-hemalum.