Mayer's Hemalum Preparation and Recipe

Table 1. Required components

Component	Amount	Concentration
aluminum potassium sulfate (KAl[SO4]2•12H2O) (mw: 474.39 g/mol)	50 g	0.1053 M
hematoxylin (C.I. 75290) (mw: 302.28 g/mol)	1 g	0.003308 M
sodium iodate (NaIO3) (mw: 197.89 g/mol)	0.1 g	0.0005053 M
citric acid (monohydrate) (mw: 210.14 g/mol)	1 g	0.004758 M
chloral hydrate (mw: 165.40 g/mol)	50 g	0.3022 M

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 50 g of aluminum potassium sulfate (KAl[SO4]2•12H2O) to the solution.
3. Add 1 g of hematoxylin (C.I. 75290) to the solution.
4. Add 0.1 g of sodium iodate (NaIO3) to the solution.
5. Add 1 g of citric acid (monohydrate) to the solution.
6. Add 50 g of chloral hydrate to the solution.
7. A magnetic stirrer will hasten dissolving of the alum, hematoxylin, and hematein formed by iodate oxidation.
8. Add distilled water until the volume is 1 L.
9. Mayer's hemalum often keeps for 1 yr but some batches lose their potency after only a few months. The solution may be reused many times.

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