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Bis-Tris Buffer (1 M, 6.5 pH) Preparation and Recipe
Bis-Tris buffer is used in molecular biology for applications involving both proteins and nucleic acids. Similar to tris-buffers, it has good buffer capacity and can be stored at room temperature. Bis-Tris interacts with several metal ions including Cu and Pb.
To prepare L of Bis-Tris Buffer (1 M, 6.5 pH):
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Table 1. Required components
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| Component | Amount | Concentration |
| Bis-Tris (mw: 209.24 g/mol) | 209.24 g | 1 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 209.24 g of Bis-Tris to the solution.
- Starting pH without HCl is 9.90. Concentrated HCl is used to adjust the pH. Filter sterilize or autoclave before use or storage.
- Add dH2O until the volume is 1 L.
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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Bis-Tris Buffer (1 M, 6.5 pH) Preparation and Recipe." AAT Bioquest, Inc., 26 Apr. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/bis-tris-buffer-ph-6-5. | |
APA | AAT Bioquest, Inc. (2024, April 26). Quest Calculate™ Bis-Tris Buffer (1 M, 6.5 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bis-tris-buffer-ph-6-5. | |
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