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Bis-Tris Buffer (1 M, 6.5 pH) Preparation and Recipe

Bis-Tris buffer is used in molecular biology for applications involving both proteins and nucleic acids. Similar to tris-buffers, it has good buffer capacity and can be stored at room temperature. Bis-Tris interacts with several metal ions including Cu and Pb.

To prepare  L of Bis-Tris Buffer (1 M, 6.5 pH):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Bis-Tris (mw: 209.24 g/mol)209.24 g1 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 209.24 g of Bis-Tris to the solution.
  3. Starting pH without HCl is 9.90. Concentrated HCl is used to adjust the pH. Filter sterilize or autoclave before use or storage.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Bis-Tris Buffer (1 M, 6.5 pH) Preparation and Recipe." AAT Bioquest, Inc.19 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/bis-tris-buffer-ph-6-5.


AAT Bioquest, Inc. (2024June 19). Quest Calculate™ Bis-Tris Buffer (1 M, 6.5 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bis-tris-buffer-ph-6-5.