PBS with Ca2+and Mg2+ (D-PBS) Preparation and Recipe
DPBS (Dulbecco's phosphate-buffered saline) with Ca2+ and Mg2+ is widely used for reagent preparation and as transport media, as well as washing and dilution.
To prepare L of PBS with Ca2+and Mg2+ (D-PBS):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Calcium Chloride (mw: 110.98 g/mol) | 0.1 g | 0.000901 M |
Potassium Chloride (mw: 74.55 g/mol) | 0.2 g | 0.002682 M |
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol) | 0.1 g | 0.0004918 M |
Sodium chloride (mw: 58.44 g/mol) | 8 g | 0.1368 M |
Sodium Phosphate Dibasic (mw: 141.96 g/mol) | 2.16 g | 0.01521 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 0.1 g of Calcium Chloride to the solution.
- Add 0.2 g of Potassium Chloride to the solution.
- Add 0.1 g of Magnesium Chloride Hexahydrate to the solution.
- Add 8 g of Sodium chloride to the solution.
- Add 2.16 g of Sodium Phosphate Dibasic to the solution.
- Add distilled water until the volume is 1 L.
- Filter-sterilize. Store at 25?C for up to 12 mo.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ PBS with Ca2+and Mg2+ (D-PBS) Preparation and Recipe." AAT Bioquest, Inc., 11 Dec. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/pbs-with-ca2-and-mg2-d-pbs. | |
APA | AAT Bioquest, Inc. (2024, December 11). Quest Calculate™ PBS with Ca2+and Mg2+ (D-PBS) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/pbs-with-ca2-and-mg2-d-pbs. | |
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This online tool has been cited in 1 publications, including
Antibacterial activity of ethanol extract and decoction from Avicennia alba Blume growing in the Can Gio Mangrove Biosphere Reserve, Vietnam
Authors:
Journal: GSC Biological and Pharmaceutical Sciences (2022): 152--159
Authors:
Journal: GSC Biological and Pharmaceutical Sciences (2022): 152--159