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PBS with Ca2+and Mg2+ (D-PBS) Preparation and Recipe

DPBS (Dulbecco's phosphate-buffered saline) with Ca2+ and Mg2+ is widely used for reagent preparation and as transport media, as well as washing and dilution.

To prepare  L of PBS with Ca2+and Mg2+ (D-PBS):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Calcium Chloride (mw: 110.98 g/mol)0.1 g0.000901 M
Potassium Chloride (mw: 74.55 g/mol)0.2 g0.002682 M
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol)0.1 g0.0004918 M
Sodium chloride (mw: 58.44 g/mol)8 g0.1368 M
Sodium Phosphate Dibasic (mw: 141.96 g/mol)2.16 g0.01521 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 0.1 g of Calcium Chloride to the solution.
  3. Add 0.2 g of Potassium Chloride to the solution.
  4. Add 0.1 g of Magnesium Chloride Hexahydrate to the solution.
  5. Add 8 g of Sodium chloride to the solution.
  6. Add 2.16 g of Sodium Phosphate Dibasic to the solution.
  7. Add distilled water until the volume is 1 L.
  8. Filter-sterilize. Store at 25?C for up to 12 mo.

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Physiological Buffer
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This online tool may be cited as follows


"Quest Calculate™ PBS with Ca2+and Mg2+ (D-PBS) Preparation and Recipe." AAT Bioquest, Inc.29 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/pbs-with-ca2-and-mg2-d-pbs.


AAT Bioquest, Inc. (2024May 29). Quest Calculate™ PBS with Ca2+and Mg2+ (D-PBS) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/pbs-with-ca2-and-mg2-d-pbs.

This online tool has been cited in 1 publications, including

Antibacterial activity of ethanol extract and decoction from Avicennia alba Blume growing in the Can Gio Mangrove Biosphere Reserve, Vietnam
Journal: GSC Biological and Pharmaceutical Sciences (2022): 152--159