AAT Bioquest

Nuclear Fast Red Solution Preparation and Recipe

Nuclear fast red is an anthrquinone dye used for nuclear staining.

To prepare  L of Nuclear Fast Red Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
nuclear fast red (Kernechtrot; C.I. 60760) (mw: 357.27 g/mol)1 g0.002799 M
aluminum sulfate (Al2[SO4]3•18H2O) (mw: 666.43 g/mol)50 g0.07503 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1 g of nuclear fast red (Kernechtrot; C.I. 60760) to the solution.
  3. Add 50 g of aluminum sulfate (Al2[SO4]3•18H2O) to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Heat with stirring until nearly boiling, then leave overnight to cool. There is a substantial residue of insoluble material, which must be removed by decanting and filtering. The solution keeps for ~1 yr; it may need to be filtered before each use.

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This online tool may be cited as follows


"Quest Calculate™ Nuclear Fast Red Solution Preparation and Recipe." AAT Bioquest, Inc.21 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/nuclear-fast-red-solution.


AAT Bioquest, Inc. (2024May 21). Quest Calculate™ Nuclear Fast Red Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/nuclear-fast-red-solution.