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MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe
MOPSO buffer is one of the Good's zwitterionic buffer with an approximate pH range of 6.5-7.9. It has a good buffering capacity but does react with iron ions. MOPSO is used for a variety of assays that require physiological pH.
To prepare
L of MOPSO Buffer (0.5 M, 6.9 pH):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
MOPSO, Free acid (mw: 225.26 g / mol)##
  • Prepare 800 mL of dH2O in a suitable container.
  • Add # of MOPSO, Free acid to the solution
  • Adjust solution to desired pH by 10N NaOH.
  • Add dH2O until the volume is 1 L.


References
This online resource may be cited as follows
MLA
"Quest CalculateMOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe." AAT Bioquest, Inc.15 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/mopso-buffer-ph-6-9.
APA
AAT Bioquest, Inc. (2025October 15). Quest CalculateMOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mopso-buffer-ph-6-9.
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