MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe

MOPSO buffer is one of the Good's zwitterionic buffer with an approximate pH range of 6.5-7.9. It has a good buffering capacity but does react with iron ions. MOPSO is used for a variety of assays that require physiological pH.

To prepare

L of MOPSO Buffer (0.5 M, 6.9 pH):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
MOPSO, Free acid (mw: 225.26 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of MOPSO, Free acid to the solution
Adjust solution to desired pH by 10N NaOH.
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe." AAT Bioquest, Inc., 15 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/mopso-buffer-ph-6-9.
APA	AAT Bioquest, Inc. (2025, October 15). Quest Calculate™ MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mopso-buffer-ph-6-9.
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