AAT Bioquest

MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe

MOPSO buffer is one of the Good's zwitterionic buffer with an approximate pH range of 6.5-7.9. It has a good buffering capacity but does react with iron ions. MOPSO is used for a variety of assays that require physiological pH.

To prepare  L of MOPSO Buffer (0.5 M, 6.9 pH):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
MOPSO, Free acid (mw: 225.26 g/mol)112.63 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 112.63 g of MOPSO, Free acid to the solution.
  3. Adjust solution to desired pH by 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe." AAT Bioquest, Inc.18 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/mopso-buffer-ph-6-9.


AAT Bioquest, Inc. (2024April 18). Quest Calculate™ MOPSO Buffer (0.5 M, 6.9 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mopso-buffer-ph-6-9.