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Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and Recipe

This low-pH buffer is used extensively with affinity chromatography, particularly with proteins and antibodies. It is also used for cell culture and other biological applications.

To prepare  L of Glycine-HCl Buffer (0.1 M, pH 3.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Glycine (mw: 75.07 g/mol)7.5 g0.1 M
Hydrochloric acid (mw: 36.46 g/mol)0.832 g0.02 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 7.5 g of Glycine to the solution.
  3. Add 0.832 g of Hydrochloric acid to the solution.
  4. Adjust solution to final desired pH using HCl or NaOH
  5. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and Recipe." AAT Bioquest, Inc.23 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-hcl-buffer-ph-3.


AAT Bioquest, Inc. (2024June 23). Quest Calculate™ Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-hcl-buffer-ph-3.