Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and Recipe

This low-pH buffer is used extensively with affinity chromatography, particularly with proteins and antibodies. It is also used for cell culture and other biological applications.

To prepare

L of Glycine-HCl Buffer (0.1 M, pH 3.0):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Glycine (mw: 75.07 g / mol)	#	#
Hydrochloric acid (mw: 36.46 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Glycine to the solution
Add # of Hydrochloric acid to the solution
Adjust solution to final desired pH using HCl or NaOH
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and Recipe." AAT Bioquest, Inc., 4 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-hcl-buffer-ph-3.
APA	AAT Bioquest, Inc. (2025, November 4). Quest Calculate™ Glycine-HCl Buffer (0.1 M, pH 3.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/glycine-hcl-buffer-ph-3.
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