EBSS (magnesium, calcium, phenol red) (pH 7.0) recipe and preparation

To prepare L of EBSS (magnesium, calcium, phenol red) (pH 7.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Calcium Chloride (anhyd.) (mw: 111 g/mol)200 mg0.001 M
Magnesium Sulfate (heptahydrate) (mw: 246 g/mol)200 mg0.0008 M
Potassium Chloride (mw: 75 g/mol)400 mg0.005 M
Sodium Bicarbonate (mw: 84 g/mol)2.2 g0.026 M
Sodium Chloride (mw: 58 g/mol)6.8 g0.117 M
Sodium Phosphate monobasic (mw: 138 g/mol)140 mg0.001 M
D-Glucose (Dextrose) (mw: 180 g/mol)1 g0.006 M
Phenol Red (mw: 354.38 g/mol)10 mg0 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 200 mg of Calcium Chloride (anhyd.) to the solution.
  3. Add 200 mg of Magnesium Sulfate (heptahydrate) to the solution.
  4. Add 400 mg of Potassium Chloride to the solution.
  5. Add 2.2 g of Sodium Bicarbonate to the solution.
  6. Add 6.8 g of Sodium Chloride to the solution.
  7. Add 140 mg of Sodium Phosphate monobasic to the solution.
  8. Add 1 g of D-Glucose (Dextrose) to the solution.
  9. Add 10 mg of Phenol Red to the solution.
  10. Adjust solution to final desired pH using HCl or NaOH
  11. Add distilled water until volume is 1 L.


Min pH

Max pH


Acetate Buffer (0.1 M, pH 5.0)3.75.6Common Physiological
BES-Buffered Saline (2X) (0.05 M, pH 6.95)6.47.8Common Physiological
Bicine (1 M, pH 8.26)7.69.0Common Physiological
CAPS (0.5 M, pH 10.4)9.711.1Common Physiological
CHES (0.5 M, pH 9.5)8.610.0Common Physiological
Citrate Buffer (0.1 M, pH 6.0)3.06.2Common Physiological
Citrate-Phosphate Buffer (0.15 M, pH 5.0)2.67.6Common Physiological
Diethanolamine (1 M, pH 9.8)7.89.9Common Physiological
EBSS (magnesium, calcium, phenol red) (pH 7.0)7.07.4Common Physiological
Glycine-HCl Buffer (0.1 M, pH 3.0)2.23.6Common Physiological
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)8.610.6Common Physiological
HBSS (Hank's Balanced Salt Solution)7.27.6Common Physiological
HEPPSO (1 M, pH 7.85)7.18.5Common Physiological
HHBS (Hank's Buffer with Hepes)7.27.4Common Physiological
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)1.02.2Common Physiological
Imidazole-HCl Buffer (0.05 M, pH 7.0)6.27.8Common Physiological
Maleic Acid (1 M, pH 7.5)7.08.0Common Physiological
MES (0.5 M, pH 6)5.56.7Common Physiological
MOPS Buffer (10X) (0.2 M, pH 7)6.57.9Common Physiological
PBS (Phosphate Buffered Saline) (1X, pH 7.4)7.37.5Common Physiological
Sodium Borate Buffer (1 M, pH 8.5)8.010.0Common Physiological
TAE (1 M, pH 8.6)8.08.5Common Physiological
TAE Buffer (50X) (0.04 M, pH 8.5)8.08.5Common Physiological
TBS (1 M, pH 7.4)7.27.5Common Physiological
TE Buffer 10X7.58.5Common Physiological
Tricine (1 M, pH 8.05)7.48.8Common Physiological
Tris Buffer (1 M, pH 7.0)7.07.5Common Physiological
Acetate Buffer (pH 3.6 to 5.6)3.75.6Variable pH
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)9.210.8Variable pH
Citrate Buffer (pH 3.0 to 6.2)3.06.2Variable pH
Phosphate Buffer (pH 5.8 to 7.4)5.88.0Variable pH
Potassium Phosphate (pH 5.8 to 8.0)5.88.0Variable pH
Trizma Buffer (pH 7.2 to 9.0)7.29.0Variable pH