EBSS (magnesium, calcium, phenol red) (pH 7.0) Preparation and Recipe

EBSS is the abbreviation of Earle's Balanced Salt Solution, and is in common use for cell culture applications including washing and dilution in CO2 environments. The addition of the magnesium and calcium ions, along with phenol red, is to assist in reagent preparation. Phenol red is a weak estrogen and should not be used long-term with responsive cell cultures. For other buffers containing Phenol Red, click here.

To prepare L of EBSS (magnesium, calcium, phenol red) (pH 7.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Calcium Chloride (mw: 110.98 g/mol)	0.2 g	0.001 M
Magesium Sulfate Heptahydrate (mw: 246.47 g/mol)	0.2 g	0.00081 M
Potassium Chloride (mw: 74.55 g/mol)	0.4 g	0.005 M
Sodium bicarbonate (mw: 84.01 g/mol)	2.2 g	0.026 M
Sodium chloride (mw: 58.44 g/mol)	6.8 g	0.117 M
Sodium Phosphate Monobasic Monohydrate (mw: 137.99 g/mol)	0.14 g	0.001 M
D-Glucose (Dextrose) (mw: 180.16 g/mol)	1 g	0.006 M
Phenol Red (mw: 354.38 g/mol)	0.01 g	0.00003 M

Prepare 800 mL of distilled water in a suitable container.
Add 0.2 g of Calcium Chloride to the solution.
Add 0.2 g of Magesium Sulfate Heptahydrate to the solution.
Add 0.4 g of Potassium Chloride to the solution.
Add 2.2 g of Sodium bicarbonate to the solution.
Add 6.8 g of Sodium chloride to the solution.
Add 0.14 g of Sodium Phosphate Monobasic Monohydrate to the solution.
Add 1 g of D-Glucose (Dextrose) to the solution.
Add 0.01 g of Phenol Red to the solution.
Adjust solution to final desired pH using HCl or NaOH
Add distilled water until the volume is 1 L.

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Physiological Buffer

pH Buffering

Sample Preparation

BioAssays

Misc

Cell/Culture/Growth Media

Gel Electrophoresis

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Extracellular Saline (1x, pH 7.3)
HBSS (Hank's Balanced Salt Solution)
HEPES Buffer (1 M, 7.5 pH)
HHBS (Hank's Buffer with Hepes)
PBS (Phosphate Buffered Saline) (1X, pH 7.4)
PBS with Ca2+and Mg2+ (D-PBS)
Tyrode's Solution, Acidic

Name ▲ ▼
ACES Buffer (0.1 M, 6.7 pH)
Acetate Buffer (0.1 M, pH 5.0)
Acetate Buffer (pH 3.6 to 5.6)
ADA Buffer (0.5 M, 6.6 pH)
Ammonium Acetate
Ammonium Bicarbonate (50 mM, pH 7.8)
Ammonium Sulfate, Saturated
BES-Buffered Saline (2X) (0.05 M, pH 6.95)
Bicine (1 M, pH 8.26)
Bis-Tris Buffer (1 M, 6.5 pH)
Borate Buffer
CAPS (0.5 M, pH 10.4)
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)
CHES (0.5 M, pH 9.5)
Citrate Buffer (0.1 M, pH 6.0)
Citrate Buffer (pH 3.0 to 6.2)
Citrate-Phosphate Buffer (0.15 M, pH 5.0)
Citrate-Phosphate Buffer (110 mM, pH 5.6)
Citrate-Phosphate Buffer (40 mM, pH 5.6)
Diethanolamine (1 M, pH 9.8)
EBSS (magnesium, calcium, phenol red) (pH 7.0)
Glycine (0.1 M, pH 2.2)
Glycine-HCl Buffer (0.1 M, pH 3.0)
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)
HEPPSO (1 M, pH 7.85)
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)
Imidazole-HCl Buffer (0.05 M, pH 7.0)
Maleic Acid (1 M, pH 7.5)
MES (0.5 M, pH 6)
MOPS Buffer (10X) (0.2 M, pH 7)
MOPSO Buffer (0.5 M, 6.9 pH)
Phosphate Buffer (pH 5.8 to 7.4)
PIPES Buffer (1 M, 6.8 pH)
Potassium Phosphate Buffer (1 M, pH 6.5)
Potassium Phosphate (pH 5.8 to 8.0)
Sodium Acetate (3 M, pH 5.2)
Sodium Borate Buffer (1 M, pH 8.5)
Sodium Phosphate Solution
SSC buffer (20X) (3 M, pH 7)
TAE (1 M, pH 8.6)
TB Buffer
TBS (1 M, pH 7.4)
TES Buffer (1 M, 7.5 pH)
Tricine (1 M, pH 8.05)
Tris Buffer (1 M, pH 7.2)
Tris-Buffered Saline (TBS, 0.1 M)
Tris-citrate Buffer (pH 8.0)
Trizma® Buffer (pH 7.0 to 9.2)

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SSPE (Blot, 20x, pH 7.4)

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Sodium Iodide/Sodium Sulfite Solution

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Bath Solution (Ca2+/Mg2+ Free)
Calcium Chloride Stock Solution
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References

This online tool may be cited as follows

MLA	"Quest Calculate™ EBSS (magnesium, calcium, phenol red) (pH 7.0) Preparation and Recipe." AAT Bioquest, Inc., 25 Apr. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ebss-calcium-magnesium-phenol-red-ph-7.
APA	AAT Bioquest, Inc. (2024, April 25). Quest Calculate™ EBSS (magnesium, calcium, phenol red) (pH 7.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ebss-calcium-magnesium-phenol-red-ph-7.
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EBSS (magnesium, calcium, phenol red) (pH 7.0) Preparation and Recipe

References

This online tool may be cited as follows

MLA

APA