ADA Buffer (0.5 M, 6.6 pH) Preparation and Recipe

ADA buffer is one of the Good's buffer with a pH range of 6.0-7.2. It is often used in cell culture media as a chelating agent for H+, Ca2+, and Mg2+.

To prepare

L of ADA Buffer (0.5 M, 6.6 pH):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
ADA (mw: 190.15 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of ADA to the solution
Add dH2O until the volume is 1 L.
ADA will not fully dissolve until the pH is raised. Adjust solution to desired pH by 10N NaOH.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ ADA Buffer (0.5 M, 6.6 pH) Preparation and Recipe." AAT Bioquest, Inc., 20 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ada-buffer-ph-6-6.
APA	AAT Bioquest, Inc. (2025, October 20). Quest Calculate™ ADA Buffer (0.5 M, 6.6 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ada-buffer-ph-6-6.
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