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ADA Buffer (0.5 M, 6.6 pH) Preparation and Recipe

ADA buffer is one of the Good's buffer with a pH range of 6.0-7.2. It is often used in cell culture media as a chelating agent for H+, Ca2+, and Mg2+.

To prepare  L of ADA Buffer (0.5 M, 6.6 pH):
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Table 1. Required components
ADA (mw: 190.15 g/mol)95.11 g0.5 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 95.11 g of ADA to the solution.
  3. Add dH2O until the volume is 1 L.
  4. ADA will not fully dissolve until the pH is raised. Adjust solution to desired pH by 10N NaOH.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ ADA Buffer (0.5 M, 6.6 pH) Preparation and Recipe." AAT Bioquest, Inc.16 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/ada-buffer-ph-6-6.


AAT Bioquest, Inc. (2024June 16). Quest Calculate™ ADA Buffer (0.5 M, 6.6 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ada-buffer-ph-6-6.