AAT Bioquest

Chelation Medium Preparation and Recipe

To prepare  L of Chelation Medium:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)40.9 g0.7 M
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol)6.1 g0.03 M
Magnesium Sulfate Heptahydrate (mw: 246.47 g/mol)7.39 g0.03 M
Potassium Chloride (mw: 74.55 g/mol)1.49 g0.02 M
EGTA (mw: 380.35 g/mol)7.6 g0.02 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 40.9 g of Sodium chloride to the solution.
  3. Add 6.1 g of Magnesium Chloride Hexahydrate to the solution.
  4. Add 7.39 g of Magnesium Sulfate Heptahydrate to the solution.
  5. Add 1.49 g of Potassium Chloride to the solution.
  6. Add 7.6 g of EGTA to the solution.
  7. Add distilled water until the volume is 1 L.
  8. Adjust pH to 5.5 using HCl. Autoclave or filter through a 0.2-µm membrane. Store at 4°C.

Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Chelation Medium Preparation and Recipe." AAT Bioquest, Inc.24 Jul2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/chelation-medium.


AAT Bioquest, Inc. (2024July 24). Quest Calculate™ Chelation Medium Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/chelation-medium.