Chelation Medium Preparation and Recipe
To prepare L of Chelation Medium:
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Sodium chloride (mw: 58.44 g/mol) | 40.9 g | 0.7 M |
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol) | 6.1 g | 0.03 M |
Magnesium Sulfate Heptahydrate (mw: 246.47 g/mol) | 7.39 g | 0.03 M |
Potassium Chloride (mw: 74.55 g/mol) | 1.49 g | 0.02 M |
EGTA (mw: 380.35 g/mol) | 7.6 g | 0.02 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 40.9 g of Sodium chloride to the solution.
- Add 6.1 g of Magnesium Chloride Hexahydrate to the solution.
- Add 7.39 g of Magnesium Sulfate Heptahydrate to the solution.
- Add 1.49 g of Potassium Chloride to the solution.
- Add 7.6 g of EGTA to the solution.
- Add distilled water until the volume is 1 L.
- Adjust pH to 5.5 using HCl. Autoclave or filter through a 0.2-µm membrane. Store at 4°C.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Chelation Medium Preparation and Recipe." AAT Bioquest, Inc., 6 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/chelation-medium. | |
APA | AAT Bioquest, Inc. (2024, October 6). Quest Calculate™ Chelation Medium Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/chelation-medium. | |
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