Products
Technologies
Applications
Services
Resources
Selection Guides
About
Chelation Medium Preparation and Recipe
To prepareL of Chelation Medium:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Sodium chloride (mw: 58.44 g / mol) | # | # |
| Magnesium Chloride Hexahydrate (mw: 203.3 g / mol) | # | # |
| Magnesium Sulfate Heptahydrate (mw: 246.47 g / mol) | # | # |
| Potassium Chloride (mw: 74.55 g / mol) | # | # |
| EGTA (mw: 380.35 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Sodium chloride to the solution
- Add # of Magnesium Chloride Hexahydrate to the solution
- Add # of Magnesium Sulfate Heptahydrate to the solution
- Add # of Potassium Chloride to the solution
- Add # of EGTA to the solution
- Add distilled water until the volume is 1 L.
- Adjust pH to 5.5 using HCl. Autoclave or filter through a 0.2-µm membrane. Store at 4°C.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ Chelation Medium Preparation and Recipe." AAT Bioquest, Inc., 23 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/chelation-medium. | |
APA | AAT Bioquest, Inc. (2025, October 23). Quest Calculate™ Chelation Medium Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/chelation-medium. | |
| BibTeX | EndNote | RefMan |