Citrate Buffer (0.1 M, pH 6.0) recipe and preparation
Citrate buffers can be used for RNA isolation, thanks to its ability to prevent base hydrolysis. It also finds use in antigen detection by breaking cross-links between antigens and any substances in its fixation medium.
To prepare L of Citrate Buffer (0.1 M, pH 6.0): Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Sodium Citrate dihydrate (mw: 294 g/mol)
Citric Acid (mw: 192.1 g/mol)
Prepare 800 mL of distilled water in a suitable container.
Add 12.044 g of Sodium Citrate dihydrate to the solution.
Add 11.341 g of Citric Acid to the solution.
Adjust solution to desired pH using 0.1N HCl (typically pH ≈ 6.0).