logo
AAT Bioquest

Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe

Citrate buffers can be used for RNA isolation, thanks to its ability to prevent base hydrolysis. It also finds use in antigen detection by breaking cross-links between antigens and any substances in its fixation medium.

Available for purchase here.

To prepare  L of Citrate Buffer (0.1 M, pH 6.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Sodium Citrate dihydrate (mw: 294.10 g/mol)24.269 g0.0825 M
Citric Acid (mw: 192.12 g/mol)3.358 g0.0175 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 24.269 g of Sodium Citrate dihydrate to the solution.
  3. Add 3.358 g of Citric Acid to the solution.
  4. Adjust solution to desired pH using 0.1N HCl (typically pH ≈ 6.0).
  5. Add distilled water until the volume is 1 L.

To make a purchase inquiry for this buffer, please provide your email address below:
Request quotation



Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe." AAT Bioquest, Inc.29 Mar2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6.

APA

AAT Bioquest, Inc. (2024March 29). Quest Calculate™ Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6.
BibTeXEndNoteRefMan

This online tool has been cited in 2 publications, including

Nephroprotective and antioxidant effects of ethanol extract of Coprinus comatus mushroom fruit-bodies on streptozotocin-induced diabetic rat models
Authors:
Journal: (2021): 012078
Factors affecting stability of RNA--temperature, length, concentration, pH, and buffering species
Authors:
Journal: Journal of Pharmaceutical Sciences (2023)