Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe

Citrate buffers can be used for RNA isolation, thanks to its ability to prevent base hydrolysis. It also finds use in antigen detection by breaking cross-links between antigens and any substances in its fixation medium.

Available for purchase here.

To prepare

L of Citrate Buffer (0.1 M, pH 6.0):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium Citrate dihydrate (mw: 294.1 g / mol)	#	#
Citric Acid (mw: 192.12 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium Citrate dihydrate to the solution
Add # of Citric Acid to the solution
Adjust solution to desired pH using 0.1N HCl (typically pH ≈ 6.0).
Add distilled water until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe." AAT Bioquest, Inc., 13 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6.
APA	AAT Bioquest, Inc. (2025, October 13). Quest Calculate™ Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6.
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This online resource has been cited in 2 publications, including

Factors affecting stability of RNA--temperature, length, concentration, pH, and buffering species

Authors:

Chheda, Urmi and Pradeepan, Selvi and Esposito, Edward and Strezsak, Steven and Fernandez-Delgado, Olivia and Kranz, James

Journal:

Journal of Pharmaceutical Sciences (2023)

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