AAT Bioquest

Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe

Citrate buffers can be used for RNA isolation, thanks to its ability to prevent base hydrolysis. It also finds use in antigen detection by breaking cross-links between antigens and any substances in its fixation medium.

Available for purchase here.

To prepare  L of Citrate Buffer (0.1 M, pH 6.0):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium Citrate dihydrate (mw: 294.10 g/mol)24.269 g0.0825 M
Citric Acid (mw: 192.12 g/mol)3.358 g0.0175 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 24.269 g of Sodium Citrate dihydrate to the solution.
  3. Add 3.358 g of Citric Acid to the solution.
  4. Adjust solution to desired pH using 0.1N HCl (typically pH ≈ 6.0).
  5. Add distilled water until the volume is 1 L.

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This online tool may be cited as follows


"Quest Calculate™ Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe." AAT Bioquest, Inc.23 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6.


AAT Bioquest, Inc. (2024June 23). Quest Calculate™ Citrate Buffer (0.1 M, pH 6.0) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/citrate-buffer-ph-6.

This online tool has been cited in 2 publications, including

Nephroprotective and antioxidant effects of ethanol extract of Coprinus comatus mushroom fruit-bodies on streptozotocin-induced diabetic rat models
Journal: (2021): 012078
Factors affecting stability of RNA--temperature, length, concentration, pH, and buffering species
Journal: Journal of Pharmaceutical Sciences (2023)