Digestion Medium Preparation and Recipe

To prepare

L of Digestion Medium:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g / mol)	#	#
Magnesium Chloride Hexahydrate (mw: 203.3 g / mol)	#	#
Magnesium Sulfate Heptahydrate (mw: 246.47 g / mol)	#	#
Potassium Chloride (mw: 74.55 g / mol)	#	#
Calcium Chloride (mw: 110.98 g / mol)	#	#
2-(N-morpholino)ethanesulfonic acid (mw: 195.24 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Sodium chloride to the solution
Add # of Magnesium Chloride Hexahydrate to the solution
Add # of Magnesium Sulfate Heptahydrate to the solution
Add # of Potassium Chloride to the solution
Add # of Calcium Chloride to the solution
Add # of 2-(N-morpholino)ethanesulfonic acid to the solution
Add distilled water until the volume is 1 L.
Adjust the pH to 6.5. Filter through a 0.2-µm filter and store at 4°C. Just before use, add 1% (w/v) cellulose (Sigma) and 3 U/mL alginate lyase (Boyen et al. 1990).

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Digestion Medium Preparation and Recipe." AAT Bioquest, Inc., 9 Nov. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/digestion-medium.
APA	AAT Bioquest, Inc. (2025, November 9). Quest Calculate™ Digestion Medium Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/digestion-medium.
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