AAT Bioquest

Digestion Medium Preparation and Recipe

To prepare  L of Digestion Medium:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)23.4 g0.4 M
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol)26.4 g0.13 M
Magnesium Sulfate Heptahydrate (mw: 246.47 g/mol)5.4 g0.022 M
Potassium Chloride (mw: 74.55 g/mol)11.9 g0.16 M
Calcium Chloride (mw: 110.98 g/mol)0.3 g0.002 M
2-(N-morpholino)ethanesulfonic acid (mw: 195.24 g/mol)1.95 g0.01 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 23.4 g of Sodium chloride to the solution.
  3. Add 26.4 g of Magnesium Chloride Hexahydrate to the solution.
  4. Add 5.4 g of Magnesium Sulfate Heptahydrate to the solution.
  5. Add 11.9 g of Potassium Chloride to the solution.
  6. Add 0.3 g of Calcium Chloride to the solution.
  7. Add 1.95 g of 2-(N-morpholino)ethanesulfonic acid to the solution.
  8. Add distilled water until the volume is 1 L.
  9. Adjust the pH to 6.5. Filter through a 0.2-µm filter and store at 4°C. Just before use, add 1% (w/v) cellulose (Sigma) and 3 U/mL alginate lyase (Boyen et al. 1990).

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This online tool may be cited as follows


"Quest Calculate™ Digestion Medium Preparation and Recipe." AAT Bioquest, Inc.23 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/digestion-medium.


AAT Bioquest, Inc. (2024June 23). Quest Calculate™ Digestion Medium Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/digestion-medium.