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Paraformaldehyde Solution (8%) Preparation and Recipe

Paraformaldehyde solution is commonly used to fix cells or tissues.

To prepare  L of Paraformaldehyde Solution (8%):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
paraformaldehyde (mw: 30.03 g/mol)80 g2.664 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 80 g of paraformaldehyde to the solution.
  3. Heat to 60°C and add 2-3 drops of 1 N NaOH.
  4. Add distilled water until the volume is 1 L.
  5. Store this stock solution at 4°C.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Paraformaldehyde Solution (8%) Preparation and Recipe." AAT Bioquest, Inc.30 Sep2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/paraformaldehyde-solution-8.

APA

AAT Bioquest, Inc. (2022September 30). Quest Calculate™ Paraformaldehyde Solution (8%) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/paraformaldehyde-solution-8.