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Paraformaldehyde Solution (8%) Preparation and Recipe

Paraformaldehyde solution is commonly used to fix cells or tissues.

To prepare  L of Paraformaldehyde Solution (8%):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
paraformaldehyde (mw: 30.03 g/mol)80 g2.664 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 80 g of paraformaldehyde to the solution.
  3. Heat to 60°C and add 2-3 drops of 1 N NaOH.
  4. Add distilled water until the volume is 1 L.
  5. Store this stock solution at 4°C.

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Physiological Buffer
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Cell/Culture/Growth Media
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This online tool may be cited as follows


"Quest Calculate™ Paraformaldehyde Solution (8%) Preparation and Recipe." AAT Bioquest, Inc.3 Feb2023


AAT Bioquest, Inc. (2023February 3). Quest Calculate™ Paraformaldehyde Solution (8%) Preparation and Recipe. AAT Bioquest.