Lysis Solution Preparation and Recipe

Table 1. Required components

Component	Amount	Concentration
Sodium chloride (mw: 58.44 g/mol)	146.1 g	2.5 M
Disodium EDTA (mw: 372.24 g/mol)	37.2 g	0.1 M
Tris base (mw: 121.14 g/mol)	1.2 g	0.01 M
Sodium Hydroxide (mw: 40.00 g/mol)	8 g	0.2 M
N-lauroylsarcosine sodium salt (mw: 293.38 g/mol)	10 g	0.03408 M

1. Prepare 800 mL of distilled water in a suitable container.
2. Add 146.1 g of Sodium chloride to the solution.
3. Add 37.2 g of Disodium EDTA to the solution.
4. Add 1.2 g of Tris base to the solution.
5. Add 8 g of Sodium Hydroxide to the solution.
6. Add 10 g of N-lauroylsarcosine sodium salt to the solution.
7. Adjust pH to 10.
8. Add distilled water until the volume is 1 L.
9. Store at room temperature.

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