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Lysis Solution Preparation and Recipe
To prepareL of Lysis Solution:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Sodium chloride (mw: 58.44 g / mol) | # | # |
| Disodium EDTA (mw: 372.24 g / mol) | # | # |
| Tris base (mw: 121.14 g / mol) | # | # |
| Sodium Hydroxide (mw: 40 g / mol) | # | # |
| N-lauroylsarcosine sodium salt (mw: 293.38 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Sodium chloride to the solution
- Add # of Disodium EDTA to the solution
- Add # of Tris base to the solution
- Add # of Sodium Hydroxide to the solution
- Add # of N-lauroylsarcosine sodium salt to the solution
- Adjust pH to 10.
- Add distilled water until the volume is 1 L.
- Store at room temperature.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ Lysis Solution Preparation and Recipe." AAT Bioquest, Inc., 18 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/lysis-stock-solution. | |
APA | AAT Bioquest, Inc. (2025, October 18). Quest Calculate™ Lysis Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/lysis-stock-solution. | |
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