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Lysis Solution Preparation and Recipe

To prepare  L of Lysis Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Sodium chloride (mw: 58.44 g/mol)146.1 g2.5 M
Disodium EDTA (mw: 372.24 g/mol)37.2 g0.1 M
Tris base (mw: 121.14 g/mol)1.2 g0.01 M
Sodium Hydroxide (mw: 40.00 g/mol)8 g0.2 M
N-lauroylsarcosine sodium salt (mw: 293.38 g/mol)10 g0.03408 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 146.1 g of Sodium chloride to the solution.
  3. Add 37.2 g of Disodium EDTA to the solution.
  4. Add 1.2 g of Tris base to the solution.
  5. Add 8 g of Sodium Hydroxide to the solution.
  6. Add 10 g of N-lauroylsarcosine sodium salt to the solution.
  7. Adjust pH to 10.
  8. Add distilled water until the volume is 1 L.
  9. Store at room temperature.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Lysis Solution Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/lysis-stock-solution.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ Lysis Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/lysis-stock-solution.