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Lysis Solution Preparation and Recipe
To prepare
L of Lysis Solution:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Sodium chloride (mw: 58.44 g / mol)##
Disodium EDTA (mw: 372.24 g / mol)##
Tris base (mw: 121.14 g / mol)##
Sodium Hydroxide (mw: 40 g / mol)##
N-lauroylsarcosine sodium salt (mw: 293.38 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Sodium chloride to the solution
  • Add # of Disodium EDTA to the solution
  • Add # of Tris base to the solution
  • Add # of Sodium Hydroxide to the solution
  • Add # of N-lauroylsarcosine sodium salt to the solution
  • Adjust pH to 10.
  • Add distilled water until the volume is 1 L.
  • Store at room temperature.


References
This online resource may be cited as follows
MLA
"Quest CalculateLysis Solution Preparation and Recipe." AAT Bioquest, Inc.18 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/lysis-stock-solution.
APA
AAT Bioquest, Inc. (2025October 18). Quest CalculateLysis Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/lysis-stock-solution.
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