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MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe

MOPS buffer is often used in polyacrylamide gel electrophoresis.

To prepare  L of MOPS Buffer (10X) (0.2 M, pH 7):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
MOPS free acid (mw: 209.26 g/mol)41.86 g0.2 M
Sodium Acetate (mw: 82.03 g/mol)4.1 g0.05 M
Disodium EDTA (mw: 372.24 g/mol)3.72 g0.01 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 41.86 g of MOPS free acid to the solution.
  3. Add 4.1 g of Sodium Acetate to the solution.
  4. Add 3.72 g of Disodium EDTA to the solution.
  5. Adjust solution to desired pH using NaOH (typical pH = 7)
  6. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe." AAT Bioquest, Inc.26 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/mops-buffer-10x-ph-7.


AAT Bioquest, Inc. (2024May 26). Quest Calculate™ MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mops-buffer-10x-ph-7.