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MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe
MOPS buffer is often used in polyacrylamide gel electrophoresis.
To prepareL of MOPS Buffer (10X) (0.2 M, pH 7):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| MOPS free acid (mw: 209.26 g / mol) | # | # |
| Sodium Acetate (mw: 82.03 g / mol) | # | # |
| Disodium EDTA (mw: 372.24 g / mol) | # | # |
- Prepare 800 mL of dH2O in a suitable container.
- Add # of MOPS free acid to the solution
- Add # of Sodium Acetate to the solution
- Add # of Disodium EDTA to the solution
- Adjust solution to desired pH using NaOH (typical pH = 7)
- Add dH2O until the volume is 1 L.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe." AAT Bioquest, Inc., 9 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/mops-buffer-10x-ph-7. | |
APA | AAT Bioquest, Inc. (2025, October 9). Quest Calculate™ MOPS Buffer (10X) (0.2 M, pH 7) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/mops-buffer-10x-ph-7. | |
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