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HEPES Buffer (1 M, 7.5 pH) Preparation and Recipe
HEPES buffer is one of the Good's zwitterionic buffer with a pH range of 6.8-8.2. It is commonly used in cell culture medium as its pH is well maintained with changes in carbon dioxide concentration.
To prepare
L of HEPES Buffer (1 M, 7.5 pH):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Hepes (mw: 238.3 g / mol)##
  • Prepare 800 mL of dH2O in a suitable container.
  • Add # of Hepes to the solution
  • Adjust solution to desired pH by 10N NaOH.
  • Add dH2O until the volume is 1 L.


References
This online resource may be cited as follows
MLA
"Quest CalculateHEPES Buffer (1 M, 7.5 pH) Preparation and Recipe." AAT Bioquest, Inc.8 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/hepes-buffer-ph-7-5.
APA
AAT Bioquest, Inc. (2025October 8). Quest CalculateHEPES Buffer (1 M, 7.5 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hepes-buffer-ph-7-5.
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