HEPES Buffer (1 M, 7.5 pH) Preparation and Recipe

HEPES buffer is one of the Good's zwitterionic buffer with a pH range of 6.8-8.2. It is commonly used in cell culture medium as its pH is well maintained with changes in carbon dioxide concentration.

To prepare

L of HEPES Buffer (1 M, 7.5 pH):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Hepes (mw: 238.3 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of Hepes to the solution
Adjust solution to desired pH by 10N NaOH.
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ HEPES Buffer (1 M, 7.5 pH) Preparation and Recipe." AAT Bioquest, Inc., 12 Jan. 2026, https://www.aatbio.com/resources/buffer-preparations-and-recipes/hepes-buffer-ph-7-5.
APA	AAT Bioquest, Inc. (2026, January 12). Quest Calculate™ HEPES Buffer (1 M, 7.5 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hepes-buffer-ph-7-5.
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