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HEPES Buffer (1 M, 7.5 pH) Preparation and Recipe

HEPES buffer is one of the Good's zwitterionic buffer with a pH range of 6.8-8.2. It is commonly used in cell culture medium as its pH is well maintained with changes in carbon dioxide concentration.

To prepare  L of HEPES Buffer (1 M, 7.5 pH):
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Table 1. Required components
Hepes (mw: 238.30 g/mol)238.3 g1 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 238.3 g of Hepes to the solution.
  3. Adjust solution to desired pH by 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ HEPES Buffer (1 M, 7.5 pH) Preparation and Recipe." AAT Bioquest, Inc.16 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/hepes-buffer-ph-7-5.


AAT Bioquest, Inc. (2024April 16). Quest Calculate™ HEPES Buffer (1 M, 7.5 pH) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/hepes-buffer-ph-7-5.