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Diethanolamine (1 M, pH 9.8) Preparation and Recipe
Diethanolamine (DEA) is the buffer of choice in applications employing concentrated Tropix substrates for the detection of alkaline phosphatase. DEA buffers provide favorable reaction kinetics and greater sensitivity compared to most buffer systems.
To prepare
L of Diethanolamine (1 M, pH 9.8):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Diethanolamine (mw: 105.14 g / mol)##
Magnesium Chloride Hexahydrate (mw: 203.3 g / mol)##
  • Prepare 800 mL of dH2O in a suitable container.
  • Add # of Diethanolamine to the solution
  • Add # of Magnesium Chloride Hexahydrate to the solution
  • Adjust the pH to 9.8 with 10 M HCl solution.
  • Add dH2O until the volume is 1 L.


References
This online resource may be cited as follows
MLA
"Quest CalculateDiethanolamine (1 M, pH 9.8) Preparation and Recipe." AAT Bioquest, Inc.13 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/diethanolamine-ph-9-8.
APA
AAT Bioquest, Inc. (2025October 13). Quest CalculateDiethanolamine (1 M, pH 9.8) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/diethanolamine-ph-9-8.
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