Diethanolamine (1 M, pH 9.8) Preparation and Recipe

Diethanolamine (DEA) is the buffer of choice in applications employing concentrated Tropix substrates for the detection of alkaline phosphatase. DEA buffers provide favorable reaction kinetics and greater sensitivity compared to most buffer systems.

To prepare

L of Diethanolamine (1 M, pH 9.8):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Diethanolamine (mw: 105.14 g / mol)	#	#
Magnesium Chloride Hexahydrate (mw: 203.3 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of Diethanolamine to the solution
Add # of Magnesium Chloride Hexahydrate to the solution
Adjust the pH to 9.8 with 10 M HCl solution.
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Diethanolamine (1 M, pH 9.8) Preparation and Recipe." AAT Bioquest, Inc., 30 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/diethanolamine-ph-9-8.
APA	AAT Bioquest, Inc. (2025, October 30). Quest Calculate™ Diethanolamine (1 M, pH 9.8) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/diethanolamine-ph-9-8.
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