Diethanolamine (1 M, pH 9.8) Preparation and Recipe
Diethanolamine (DEA) is the buffer of choice in applications employing concentrated Tropix substrates for the detection of alkaline phosphatase. DEA buffers provide favorable reaction kinetics and greater sensitivity compared to most buffer systems.
To prepare L of Diethanolamine (1 M, pH 9.8):
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Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Diethanolamine (mw: 105.14 g/mol) | 105.14 g | 1 M |
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol) | 0.1 g | 0.0005 M |
- Prepare 800 mL of dH2O in a suitable container.
- Add 105.14 g of Diethanolamine to the solution.
- Add 0.1 g of Magnesium Chloride Hexahydrate to the solution.
- Adjust the pH to 9.8 with 10 M HCl solution.
- Add dH2O until the volume is 1 L.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Diethanolamine (1 M, pH 9.8) Preparation and Recipe." AAT Bioquest, Inc., 11 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/diethanolamine-ph-9-8. | |
APA | AAT Bioquest, Inc. (2024, October 11). Quest Calculate™ Diethanolamine (1 M, pH 9.8) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/diethanolamine-ph-9-8. | |
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