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Diethanolamine (1 M, pH 9.8) Preparation and Recipe

Diethanolamine (DEA) is the buffer of choice in applications employing concentrated Tropix substrates for the detection of alkaline phosphatase. DEA buffers provide favorable reaction kinetics and greater sensitivity compared to most buffer systems.

To prepare  L of Diethanolamine (1 M, pH 9.8):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Diethanolamine (mw: 105.14 g/mol)105.14 g1 M
Magnesium Chloride Hexahydrate (mw: 203.30 g/mol)0.1 g0.0005 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 105.14 g of Diethanolamine to the solution.
  3. Add 0.1 g of Magnesium Chloride Hexahydrate to the solution.
  4. Adjust the pH to 9.8 with 10 M HCl solution.
  5. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Diethanolamine (1 M, pH 9.8) Preparation and Recipe." AAT Bioquest, Inc.29 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/diethanolamine-ph-9-8.


AAT Bioquest, Inc. (2024May 29). Quest Calculate™ Diethanolamine (1 M, pH 9.8) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/diethanolamine-ph-9-8.