Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes Preparation and Recipe

Amino acid dropout mixtures contain amino acid supplements necessary for the growth of yeast. This amino acid dropout mixture is suitable for use with yeast-two hybrid systems.

To prepare

L of Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Adenine (mw: 135.13 g / mol)	#	#
L-Arginine (mw: 174.2 g / mol)	#	#
L-Aspartic acid (mw: 133.1 g / mol)	#	#
L-Histidine (mw: 155.15 g / mol)	#	#
L-Isoleucine (mw: 131.17 g / mol)	#	#
L-Leucine (mw: 131.17 g / mol)	#	#
L-Lysine (mw: 146.19 g / mol)	#	#
L-Methionine (mw: 149.21 g / mol)	#	#
L-Phenylalanine (mw: 165.19 g / mol)	#	#
L-Threonine (mw: 119.12 g / mol)	#	#
L-Tryptophan (mw: 204.23 g / mol)	#	#
L-Tyrosine (mw: 181.19 g / mol)	#	#
L-Valine (mw: 117.15 g / mol)	#	#
Uracil (mw: 112.09 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of Adenine to the solution
Add # of L-Arginine to the solution
Add # of L-Aspartic acid to the solution
Add # of L-Histidine to the solution
Add # of L-Isoleucine to the solution
Add # of L-Leucine to the solution
Add # of L-Lysine to the solution
Add # of L-Methionine to the solution
Add # of L-Phenylalanine to the solution
Add # of L-Threonine to the solution
Add # of L-Tryptophan to the solution
Add # of L-Tyrosine to the solution
Add # of L-Valine to the solution
Add # of Uracil to the solution
Add distilled water until the volume is 1 L.
Combine the appropriate ingredients, and mix in a sealed container. Omit the desired dropout component. Haploid yeast requires a single dropout (i.e., -Trp=-T or -Leu=-L); diploid yeast requires double dropouts (i.e., -Leu -Trp=-LT); and selecting for interactions requires triple dropouts (i.e., -Leu-Trp -His=-LTH, plus 3-AT).

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes Preparation and Recipe." AAT Bioquest, Inc., 7 Dec. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/amino-acid-dropout-mixture-for-y2h.
APA	AAT Bioquest, Inc. (2025, December 7). Quest Calculate™ Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/amino-acid-dropout-mixture-for-y2h.
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