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Tris-Buffered Saline (TBS, 0.1 M) Preparation and Recipe
Tris-Buffered Saline (TBS) is commonly used for washing in many applications, such as cell culture, IHC, WB, and ELISA.
To prepareL of Tris-Buffered Saline (TBS, 0.1 M):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Sodium chloride (mw: 58.44 g / mol) | # | # |
| Potassium Chloride (mw: 74.55 g / mol) | # | # |
| Tris (mw: 121.14 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Sodium chloride to the solution
- Add # of Potassium Chloride to the solution
- Add # of Tris to the solution
- Adjust the pH to 7.4 with HCl.
- Add distilled water until the volume is 1 L.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ Tris-Buffered Saline (TBS, 0.1 M) Preparation and Recipe." AAT Bioquest, Inc., 10 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffered-saline-tbs-0-1-m. | |
APA | AAT Bioquest, Inc. (2025, October 10). Quest Calculate™ Tris-Buffered Saline (TBS, 0.1 M) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffered-saline-tbs-0-1-m. | |
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