AAT Bioquest

Tris-Buffered Saline (TBS, 0.1 M) Preparation and Recipe

Tris-Buffered Saline (TBS) is commonly used for washing in many applications, such as cell culture, IHC, WB, and ELISA.

To prepare  L of Tris-Buffered Saline (TBS, 0.1 M):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Sodium chloride (mw: 58.44 g/mol)8 g0.1368 M
Potassium Chloride (mw: 74.55 g/mol)0.2 g0.002682 M
Tris (mw: 121.14 g/mol)12.1 g0.09988 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 8 g of Sodium chloride to the solution.
  3. Add 0.2 g of Potassium Chloride to the solution.
  4. Add 12.1 g of Tris to the solution.
  5. Adjust the pH to 7.4 with HCl.
  6. Add distilled water until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ Tris-Buffered Saline (TBS, 0.1 M) Preparation and Recipe." AAT Bioquest, Inc.19 Apr2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffered-saline-tbs-0-1-m.


AAT Bioquest, Inc. (2024April 19). Quest Calculate™ Tris-Buffered Saline (TBS, 0.1 M) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tris-buffered-saline-tbs-0-1-m.