AAT Bioquest

Bicine (1 M, pH 8.26) Preparation and Recipe

Bicine is used in peptide and protein biochemical applications, and is ideal for low temperatures. Bicine is a Zwitterionic compound, also known as a dipolar ion.

To prepare  L of Bicine (1 M, pH 8.26):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Bicine (mw: 163.17 g/mol)163.17 g1 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 163.17 g of Bicine to the solution.
  3. Adjust solution to desired pH with 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
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This online tool may be cited as follows


"Quest Calculate™ Bicine (1 M, pH 8.26) Preparation and Recipe." AAT Bioquest, Inc.16 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/bicine-ph-8-26.


AAT Bioquest, Inc. (2024June 16). Quest Calculate™ Bicine (1 M, pH 8.26) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bicine-ph-8-26.