Bicine (1 M, pH 8.26) Preparation and Recipe

Bicine is used in peptide and protein biochemical applications, and is ideal for low temperatures. Bicine is a Zwitterionic compound, also known as a dipolar ion.

To prepare

L of Bicine (1 M, pH 8.26):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Bicine (mw: 163.17 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of Bicine to the solution
Adjust solution to desired pH with 10N NaOH.
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Bicine (1 M, pH 8.26) Preparation and Recipe." AAT Bioquest, Inc., 13 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/bicine-ph-8-26.
APA	AAT Bioquest, Inc. (2025, October 13). Quest Calculate™ Bicine (1 M, pH 8.26) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bicine-ph-8-26.
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