AAT Bioquest

Ammonium Sulfate, Saturated Preparation and Recipe

Ammonium Sulfate is commonly used for protein precipitation and fractionation due to enhancing hydrophobic interactions.

To prepare  L of Ammonium Sulfate, Saturated:
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Table 1. Required components
ammonium sulfate (mw: 132.14 g/mol)761 g5.759 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 761 g of ammonium sulfate to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Dissolve with stirring and gentle heating. Cool to room temperature and adjust the pH to 7 (with NH4OH or an acid such as HCl). If excess is to be stored, sterilize the solution by filtering through a 0.2-µm filter and store at 4°C.

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Physiological Buffer
pH Buffering
Sample Preparation
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This online tool may be cited as follows


"Quest Calculate™ Ammonium Sulfate, Saturated Preparation and Recipe." AAT Bioquest, Inc.16 Jun2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/ammonium-sulfate-saturated.


AAT Bioquest, Inc. (2024June 16). Quest Calculate™ Ammonium Sulfate, Saturated Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ammonium-sulfate-saturated.