Actively helping customers, employees and the global community during the coronavirus SARS-CoV-2 outbreak.  Learn more >>

Ammonium Sulfate, Saturated Preparation and Recipe

Ammonium Sulfate is commonly used for protein precipitation and fractionation due to enhancing hydrophobic interactions.

To prepare  L of Ammonium Sulfate, Saturated:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
ammonium sulfate (mw: 132.14 g/mol)761 g5.759 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 761 g of ammonium sulfate to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Dissolve with stirring and gentle heating. Cool to room temperature and adjust the pH to 7 (with NH4OH or an acid such as HCl). If excess is to be stored, sterilize the solution by filtering through a 0.2-µm filter and store at 4°C.

To make a purchase inquiry for this buffer, please provide your email address below:
Request quotation



Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ Ammonium Sulfate, Saturated Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/ammonium-sulfate-saturated.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ Ammonium Sulfate, Saturated Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ammonium-sulfate-saturated.