Ammonium Sulfate, Saturated Preparation and Recipe

Ammonium Sulfate is commonly used for protein precipitation and fractionation due to enhancing hydrophobic interactions.

To prepare

L of Ammonium Sulfate, Saturated:

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
ammonium sulfate (mw: 132.14 g / mol)	#	#

Prepare 800 mL of distilled water in a suitable container.
Add # of ammonium sulfate to the solution
Add distilled water until the volume is 1 L.
Dissolve with stirring and gentle heating. Cool to room temperature and adjust the pH to 7 (with NH4OH or an acid such as HCl). If excess is to be stored, sterilize the solution by filtering through a 0.2-µm filter and store at 4°C.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ Ammonium Sulfate, Saturated Preparation and Recipe." AAT Bioquest, Inc., 21 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/ammonium-sulfate-saturated.
APA	AAT Bioquest, Inc. (2025, October 21). Quest Calculate™ Ammonium Sulfate, Saturated Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/ammonium-sulfate-saturated.
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