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BAC DNA Microinjection Buffer Preparation and Recipe
BAC injection buffer functions to stabilize BAC DNA in preparation for microinjection.
To prepare
L of BAC DNA Microinjection Buffer:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Tris (pH 7.5) (mw: 121.14 g / mol)##
EDTA (mw: 292.24 g / mol)##
Sodium chloride (mw: 58.44 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Tris (pH 7.5) to the solution
  • Add # of EDTA to the solution
  • Add # of Sodium chloride to the solution
  • Adjust the pH to 7.4.
  • Add distilled water until the volume is 1 L.
  • Filter the injection buffer (through a 0.2-U filter) and store at 4?C.
References
This online resource may be cited as follows
MLA
"Quest Calculate™ BAC DNA Microinjection Buffer Preparation and Recipe." AAT Bioquest, Inc., 22 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/bac-injection-buffer.
APA
AAT Bioquest, Inc. (2025, October 22). Quest Calculate™ BAC DNA Microinjection Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bac-injection-buffer.
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