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BAC DNA Microinjection Buffer Preparation and Recipe

BAC injection buffer functions to stabilize BAC DNA in preparation for microinjection.

To prepare  L of BAC DNA Microinjection Buffer:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Tris (pH 7.5) (mw: 121.14 g/mol)1.211 g0.01 M
EDTA (mw: 292.24 g/mol)0.02922 g0.0001 M
Sodium chloride (mw: 58.44 g/mol)5.844 g0.1 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1.211 g of Tris (pH 7.5) to the solution.
  3. Add 0.02922 g of EDTA to the solution.
  4. Add 5.844 g of Sodium chloride to the solution.
  5. Adjust the pH to 7.4.
  6. Add distilled water until the volume is 1 L.
  7. Filter the injection buffer (through a 0.2-U filter) and store at 4?C.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ BAC DNA Microinjection Buffer Preparation and Recipe." AAT Bioquest, Inc.30 Sep2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/bac-injection-buffer.

APA

AAT Bioquest, Inc. (2022September 30). Quest Calculate™ BAC DNA Microinjection Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bac-injection-buffer.