H-50 Buffer Preparation and Recipe
H-50 buffer is commonly used for washing and resuspending cells. It contains HEPES, one of the Good's zwitterionic buffers.
To prepare L of H-50 Buffer:
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
Hepes (mw: 238.30 g/mol) | 4.76 g | 0.02 M |
Potassium Chloride (mw: 74.55 g/mol) | 3.73 g | 0.05 M |
Magnesium sulfate (mw: 120.37 g/mol) | 0.12 g | 0.001 M |
Sodium chloride (mw: 58.44 g/mol) | 0.58 g | 0.01 M |
Sodium bicarbonate (mw: 84.01 g/mol) | 0.42 g | 0.005 M |
Sodium phoshpate monobasic dihydrate (mw: 156.01 g/mol) | 0.156 g | 0.001 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 4.76 g of Hepes to the solution.
- Add 3.73 g of Potassium Chloride to the solution.
- Add 0.12 g of Magnesium sulfate to the solution.
- Add 0.58 g of Sodium chloride to the solution.
- Add 0.42 g of Sodium bicarbonate to the solution.
- Add 0.156 g of Sodium phoshpate monobasic dihydrate to the solution.
- Add distilled water until the volume is 1 L.
- Adjust the pH to 7.0 with HCl or NaOH, as appropriate. Autoclave and store cold or frozen.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ H-50 Buffer Preparation and Recipe." AAT Bioquest, Inc., 5 Oct. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/h-50-buffer. | |
APA | AAT Bioquest, Inc. (2024, October 5). Quest Calculate™ H-50 Buffer Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/h-50-buffer. | |
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