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EDTA Solution Preparation and Recipe

Metal Stripping Solution is comprised of EDTA, which can act as a metal chelating agent.

To prepare  L of EDTA Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
EDTA (mw: 292.24 g/mol)11.7 g0.04003 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 11.7 g of EDTA to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Slowly adjust the pH to 8.0 with 2 M NaOH. (Unless using the salt, EDTA often requires extended time and basic pH conditions to completely dissolve.) Store for up to 3 mo at 4°C.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ EDTA Solution Preparation and Recipe." AAT Bioquest, Inc.1 Oct2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/edta-solution.

APA

AAT Bioquest, Inc. (2022October 1). Quest Calculate™ EDTA Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/edta-solution.