EDTA Solution Preparation and Recipe

Metal Stripping Solution is comprised of EDTA, which can act as a metal chelating agent.

To prepare L of EDTA Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
EDTA (mw: 292.24 g/mol)	11.7 g	0.04003 M

Prepare 800 mL of distilled water in a suitable container.
Add 11.7 g of EDTA to the solution.
Add distilled water until the volume is 1 L.
Slowly adjust the pH to 8.0 with 2 M NaOH. (Unless using the salt, EDTA often requires extended time and basic pH conditions to completely dissolve.) Store for up to 3 mo at 4°C.

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Physiological Buffer

pH Buffering

Sample Preparation

BioAssays

Misc

Cell/Culture/Growth Media

Gel Electrophoresis

Name ▲ ▼
PBS (Phosphate Buffered Saline) (1X, pH 7.4)
HBSS (Hank's Balanced Salt Solution)
HHBS (Hank's Buffer with Hepes)
HEPES Buffer (1 M, 7.5 pH)
PBS with Ca2+and Mg2+ (D-PBS)
Extracellular Saline (1x, pH 7.3)
Tyrode's Solution, Acidic

Name ▲ ▼
Citrate Buffer (pH 3.0 to 6.2)
Acetate Buffer (pH 3.6 to 5.6)
Tris Buffer (1 M, pH 7.2)
Citrate Buffer (0.1 M, pH 6.0)
Acetate Buffer (0.1 M, pH 5.0)
Phosphate Buffer (pH 5.8 to 7.4)
Hydrochloric Acid-Potassium Chloride Buffer (0.1 M, pH 2.0)
Glycine-HCl Buffer (0.1 M, pH 3.0)
Citrate-Phosphate Buffer (0.15 M, pH 5.0)
Glycine-Sodium Hydroxide Buffer (0.08 M, pH 10)
EBSS (magnesium, calcium, phenol red) (pH 7.0)
Imidazole-HCl Buffer (0.05 M, pH 7.0)
Trizma® Buffer (pH 7.0 to 9.2)
MES (0.5 M, pH 6)
Bis-Tris Buffer (1 M, 6.5 pH)
ADA Buffer (0.5 M, 6.6 pH)
ACES Buffer (0.1 M, 6.7 pH)
PIPES Buffer (1 M, 6.8 pH)
MOPSO Buffer (0.5 M, 6.9 pH)
BES-Buffered Saline (2X) (0.05 M, pH 6.95)
TES Buffer (1 M, 7.5 pH)
MOPS Buffer (10X) (0.2 M, pH 7)
SSC buffer (20X) (3 M, pH 7)
TBS (1 M, pH 7.4)
Bicine (1 M, pH 8.26)
Tricine (1 M, pH 8.05)
TAE (1 M, pH 8.6)
CAPS (0.5 M, pH 10.4)
HEPPSO (1 M, pH 7.85)
CHES (0.5 M, pH 9.5)
Potassium Phosphate (pH 5.8 to 8.0)
Diethanolamine (1 M, pH 9.8)
Sodium Borate Buffer (1 M, pH 8.5)
Maleic Acid (1 M, pH 7.5)
Tris-Buffered Saline (TBS, 0.1 M)
Ammonium Sulfate, Saturated
Potassium Phosphate Buffer (1 M, pH 6.5)
Citrate-Phosphate Buffer (110 mM, pH 5.6)
Citrate-Phosphate Buffer (40 mM, pH 5.6)
Sodium Phosphate Solution
Sodium Acetate (3 M, pH 5.2)
TB Buffer
Tris-citrate Buffer (pH 8.0)
Borate Buffer
Glycine (0.1 M, pH 2.2)
Ammonium Bicarbonate (50 mM, pH 7.8)
Ammonium Acetate
Carbonate-Bicarbonate Buffer (pH 9.2 to 10.6)

Name ▲ ▼
TE Buffer 10X
Elution Buffer
BAC DNA Microinjection Buffer
EDTA Solution
ACK Lysis Buffer
Paraformaldehyde Solution (8%)
Gey's Balanced Salt Solution
Lysis Solution
H-50 Buffer
Alkaline Sodium Thiosulfate
Osmium Tetroxide Solution
DEAE-dextran
Trichloroacetic Acid (TCA)

Name ▲ ▼
Bradford Stock Solution
Anesthetic (MS222) Solution
5-Fluorocytosine (5-FC) Solution
SSC (Northern Blot, 20x)
SSPE (Blot, 20x, pH 7.4)
Immunofluorescence Quenching Solution
Nuclear Fast Red Solution
Pyronin Y (PY) Solution
Mayer's Hemalum
Chloroquine
Ethidium Bromide Solution
Cresol Red Solution

Name ▲ ▼
Sodium Iodide/Sodium Sulfite Solution
Dithiothreitol (DTT) (1 M)

Name ▲ ▼
Bath Solution (Ca2+/Mg2+ Free)
Modified Barth's Saline (MBS)
Calcium Chloride Stock Solution
Yeast Two Hybrid (Y2H) Media, Amino Acid Dropout Mixes
Digestion Medium
Chelation Medium
Penicillin/Streptomycin/Chloramphenicol Antibiotic Mix
Embryonic Medium
Carbenicillin Solution
M9 Minimal Salts
IPTG Solution

Name ▲ ▼
SDS-PAGE SDS Running Buffer (10x)
Electrophoretic Transfer Buffer (20x)
Tris-Glycine Transfer Buffer (20x)
Sodium Carbonate Transfer Buffer (40x, pH 9.5)
Acrylamide Stock Solution
Imidazole-SDS Solution
Electrode Buffer
Tris/EACA Buffer

References

MLA	"Quest Calculate™ EDTA Solution Preparation and Recipe." AAT Bioquest, Inc., 3 Feb. 2023, https://www.aatbio.com/resources/buffer-preparations-and-recipes/edta-solution.
APA	AAT Bioquest, Inc. (2023, February 3). Quest Calculate™ EDTA Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/edta-solution.

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