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BES-Buffered Saline (2X) (0.05 M, pH 6.95) Preparation and Recipe
BES-buffered saline is often used in the calcium phosphate-mediated transformation of mammalian cells.
To prepareL of BES-Buffered Saline (2X) (0.05 M, pH 6.95):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| BES (mw: 213.25 g / mol) | # | # |
| Sodium chloride (mw: 58.44 g / mol) | # | # |
| Sodium Phosphate Dibasic (mw: 141.96 g / mol) | # | # |
- Prepare 800 mL of dH2O in a suitable container.
- Add # of BES to the solution
- Add # of Sodium chloride to the solution
- Add # of Sodium Phosphate Dibasic to the solution
- Adjust solution to desired pH using 1M NaOH (typical pH = 6.95)
- Add dH2O until the volume is 1 L.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ BES-Buffered Saline (2X) (0.05 M, pH 6.95) Preparation and Recipe." AAT Bioquest, Inc., 21 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/bes-buffered-saline-2x-ph-6-95. | |
APA | AAT Bioquest, Inc. (2025, October 21). Quest Calculate™ BES-Buffered Saline (2X) (0.05 M, pH 6.95) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bes-buffered-saline-2x-ph-6-95. | |
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