AAT Bioquest

BES-Buffered Saline (2X) (0.05 M, pH 6.95) Preparation and Recipe

BES-buffered saline is often used in the calcium phosphate-mediated transformation of mammalian cells.

To prepare  L of BES-Buffered Saline (2X) (0.05 M, pH 6.95):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
BES (mw: 213.25 g/mol)10.66 g0.05 M
Sodium chloride (mw: 58.44 g/mol)16.36 g0.28 M
Sodium Phosphate Dibasic (mw: 141.96 g/mol)0.21 g0.0015 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 10.66 g of BES to the solution.
  3. Add 16.36 g of Sodium chloride to the solution.
  4. Add 0.21 g of Sodium Phosphate Dibasic to the solution.
  5. Adjust solution to desired pH using 1M NaOH (typical pH = 6.95)
  6. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ BES-Buffered Saline (2X) (0.05 M, pH 6.95) Preparation and Recipe." AAT Bioquest, Inc.28 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/bes-buffered-saline-2x-ph-6-95.


AAT Bioquest, Inc. (2024May 28). Quest Calculate™ BES-Buffered Saline (2X) (0.05 M, pH 6.95) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/bes-buffered-saline-2x-ph-6-95.