Osmium Tetroxide Solution Preparation and Recipe
Osmium tetroxide solution is used as a fixative in electron microscopy.
To prepare L of Osmium Tetroxide Solution:
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
osmium tetroxide (mw: 254.23 g/mol) | 40 g | 0.1573 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 40 g of osmium tetroxide to the solution.
- Add distilled water until the volume is 1 L.
- Make sure that the lid is tight and well sealed. Wrap in aluminum foil and stand the bottle in a larger container, sealed with a screw cap. Leave the osmium tetroxide to dissolve for 2-3 days at 4°C. Dilute 1 in 5 in double-distilled H2O before use. Osmium tetroxide solutions may be stored at -20°C for up to 6 months.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Osmium Tetroxide Solution Preparation and Recipe." AAT Bioquest, Inc., 10 Dec. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/osmium-tetroxide-solution. | |
APA | AAT Bioquest, Inc. (2024, December 10). Quest Calculate™ Osmium Tetroxide Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/osmium-tetroxide-solution. | |
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