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Osmium Tetroxide Solution Preparation and Recipe

Osmium tetroxide solution is used as a fixative in electron microscopy.

To prepare  L of Osmium Tetroxide Solution:
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Table 1. Required components
osmium tetroxide (mw: 254.23 g/mol)40 g0.1573 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 40 g of osmium tetroxide to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Make sure that the lid is tight and well sealed. Wrap in aluminum foil and stand the bottle in a larger container, sealed with a screw cap. Leave the osmium tetroxide to dissolve for 2-3 days at 4°C. Dilute 1 in 5 in double-distilled H2O before use. Osmium tetroxide solutions may be stored at -20°C for up to 6 months.

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This online tool may be cited as follows


"Quest Calculate™ Osmium Tetroxide Solution Preparation and Recipe." AAT Bioquest, Inc.24 May2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/osmium-tetroxide-solution.


AAT Bioquest, Inc. (2024May 24). Quest Calculate™ Osmium Tetroxide Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/osmium-tetroxide-solution.