AAT Bioquest

HEPPSO (1 M, pH 7.85) Preparation and Recipe

HEPPSO is a Good's buffer used with protein separation in biological research. It is a Zwitterionic compound and binds strongly with copper ions.

To prepare  L of HEPPSO (1 M, pH 7.85):
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Table 1. Required components
HEPPSO (mw: 286.349 g/mol)268.33 g1 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 268.33 g of HEPPSO to the solution.
  3. Adjust solution to desired pH with 10N NaOH.
  4. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
Cell/Culture/Growth Media
Gel Electrophoresis


This online tool may be cited as follows


"Quest Calculate™ HEPPSO (1 M, pH 7.85) Preparation and Recipe." AAT Bioquest, Inc.9 Jun2023https://www.aatbio.com/resources/buffer-preparations-and-recipes/heppso-ph-7-85.


AAT Bioquest, Inc. (2023June 9). Quest Calculate™ HEPPSO (1 M, pH 7.85) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/heppso-ph-7-85.