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HEPPSO (1 M, pH 7.85) Preparation and Recipe
HEPPSO is a Good's buffer used with protein separation in biological research. It is a Zwitterionic compound and binds strongly with copper ions.
To prepare
L of HEPPSO (1 M, pH 7.85):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
HEPPSO (mw: 286.349 g / mol)##
  • Prepare 800 mL of dH2O in a suitable container.
  • Add # of HEPPSO to the solution
  • Adjust solution to desired pH with 10N NaOH.
  • Add dH2O until the volume is 1 L.
References
This online resource may be cited as follows
MLA
"Quest Calculate™ HEPPSO (1 M, pH 7.85) Preparation and Recipe." AAT Bioquest, Inc., 16 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/heppso-ph-7-85.
APA
AAT Bioquest, Inc. (2025, October 16). Quest Calculate™ HEPPSO (1 M, pH 7.85) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/heppso-ph-7-85.
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