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Immunofluorescence Quenching Solution Preparation and Recipe
Quenching solution is used in immunoflorescence and immunocytochemistry applications.
To prepare
L of Immunofluorescence Quenching Solution:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
Glycine (mw: 75.07 g / mol)##
Ammonium chloride (mw: 53.49 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of Glycine to the solution
  • Add # of Ammonium chloride to the solution
  • Add distilled water until the volume is 1 L.
  • Quenching solution can be stored at ~20°C for several weeks.


References
This online resource may be cited as follows
MLA
"Quest CalculateImmunofluorescence Quenching Solution Preparation and Recipe." AAT Bioquest, Inc.8 Oct2025https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution.
APA
AAT Bioquest, Inc. (2025October 8). Quest CalculateImmunofluorescence Quenching Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution.
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