Immunofluorescence Quenching Solution Preparation and Recipe
Quenching solution is used in immunoflorescence and immunocytochemistry applications.
To prepare L of Immunofluorescence Quenching Solution:
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
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Component | Amount | Concentration |
Glycine (mw: 75.07 g/mol) | 3.75 g | 0.05 M |
Ammonium chloride (mw: 53.49 g/mol) | 2.67 g | 0.05 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 3.75 g of Glycine to the solution.
- Add 2.67 g of Ammonium chloride to the solution.
- Add distilled water until the volume is 1 L.
- Quenching solution can be stored at ~20°C for several weeks.
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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Immunofluorescence Quenching Solution Preparation and Recipe." AAT Bioquest, Inc., 30 May. 2023, https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution. |
APA | AAT Bioquest, Inc. (2023, May 30). Quest Calculate™ Immunofluorescence Quenching Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution. |