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Immunofluorescence Quenching Solution Preparation and Recipe
Quenching solution is used in immunoflorescence and immunocytochemistry applications.
To prepareL of Immunofluorescence Quenching Solution:
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
| Component | Amount | Concentration |
|---|---|---|
| Glycine (mw: 75.07 g / mol) | # | # |
| Ammonium chloride (mw: 53.49 g / mol) | # | # |
- Prepare 800 mL of distilled water in a suitable container.
- Add # of Glycine to the solution
- Add # of Ammonium chloride to the solution
- Add distilled water until the volume is 1 L.
- Quenching solution can be stored at ~20°C for several weeks.
References
This online resource may be cited as follows
MLA | "Quest Calculate™ Immunofluorescence Quenching Solution Preparation and Recipe." AAT Bioquest, Inc., 22 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution. | |
APA | AAT Bioquest, Inc. (2025, October 22). Quest Calculate™ Immunofluorescence Quenching Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution. | |
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