AAT Bioquest

Immunofluorescence Quenching Solution Preparation and Recipe

Quenching solution is used in immunoflorescence and immunocytochemistry applications.

To prepare  L of Immunofluorescence Quenching Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
Glycine (mw: 75.07 g/mol)3.75 g0.05 M
Ammonium chloride (mw: 53.49 g/mol)2.67 g0.05 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 3.75 g of Glycine to the solution.
  3. Add 2.67 g of Ammonium chloride to the solution.
  4. Add distilled water until the volume is 1 L.
  5. Quenching solution can be stored at ~20°C for several weeks.

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This online tool may be cited as follows


"Quest Calculate™ Immunofluorescence Quenching Solution Preparation and Recipe." AAT Bioquest, Inc.30 May2023https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution.


AAT Bioquest, Inc. (2023May 30). Quest Calculate™ Immunofluorescence Quenching Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/quenching-solution.