TAE (1 M, pH 8.6) Preparation and Recipe

TAE buffer is commonly used with nucleic acids for agarose electrophoresis applications. For the associated fluorescent probes for gel electrophoresis, click here.

To prepare

L of TAE (1 M, pH 8.6):

Change the value in the textbox above to scale the recipe volume

Table 1. Required components

Component	Amount	Concentration
Tris base (mw: 121.14 g / mol)	#	#
Disodium EDTA (mw: 372.24 g / mol)	#	#
Acetic Acid (mw: 60.05 g / mol)	#	#

Prepare 800 mL of dH2O in a suitable container.
Add # of Tris base to the solution
Add # of Disodium EDTA to the solution
Add # of Acetic Acid to the solution
The 1x TAE solution is 40mM Tris, 20mM Acetate and 1mM EDTA and typically has a pH around 8.6 (do not adjust).
Add dH2O until the volume is 1 L.

References

This online resource may be cited as follows

MLA	"Quest Calculate™ TAE (1 M, pH 8.6) Preparation and Recipe." AAT Bioquest, Inc., 9 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/tae-ph-8-6.
APA	AAT Bioquest, Inc. (2025, October 9). Quest Calculate™ TAE (1 M, pH 8.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tae-ph-8-6.
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