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TAE (1 M, pH 8.6) Preparation and Recipe

TAE buffer is commonly used with nucleic acids for agarose electrophoresis applications. For the associated fluorescent probes for gel electrophoresis, click here.

To prepare  L of TAE (1 M, pH 8.6):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
ComponentAmountConcentration
Tris base (mw: 121.14 g/mol)242 g2 M
Disodium EDTA (mw: 372.24 g/mol)18.61 g1 M
Acetic Acid (mw: 60.05 g/mol)59.955 g1 M
  1. Prepare 800 mL of dH2O in a suitable container.
  2. Add 242 g of Tris base to the solution.
  3. Add 18.61 g of Disodium EDTA to the solution.
  4. Add 59.955 g of Acetic Acid to the solution.
  5. The 1x TAE solution is 40mM Tris, 20mM Acetate and 1mM EDTA and typically has a pH around 8.6 (do not adjust).
  6. Add dH2O until the volume is 1 L.

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Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis


References

This online tool may be cited as follows

MLA

"Quest Calculate™ TAE (1 M, pH 8.6) Preparation and Recipe." AAT Bioquest, Inc.6 Dec2022https://www.aatbio.com/resources/buffer-preparations-and-recipes/tae-ph-8-6.

APA

AAT Bioquest, Inc. (2022December 6). Quest Calculate™ TAE (1 M, pH 8.6) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/tae-ph-8-6.