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Dithiothreitol (DTT) (1 M) Preparation and Recipe
DTT (Dithiothreitol) is a commonly used reducing agent.
To prepare
L of Dithiothreitol (DTT) (1 M):
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
ComponentAmountConcentration
DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) (mw: 154.25 g / mol)##
  • Prepare 800 mL of distilled water in a suitable container.
  • Add # of DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) to the solution
  • Add distilled water until the volume is 1 L.
  • Dispense into 1-mL aliquots, and store them in the dark (wrapped in aluminum foil) at -20°C (indefinitely). Do not autoclave DTT or solutions containing it.
References
This online resource may be cited as follows
MLA
"Quest Calculate™ Dithiothreitol (DTT) (1 M) Preparation and Recipe." AAT Bioquest, Inc., 14 Oct. 2025, https://www.aatbio.com/resources/buffer-preparations-and-recipes/dithiothreitol-dtt-1-m.
APA
AAT Bioquest, Inc. (2025, October 14). Quest Calculate™ Dithiothreitol (DTT) (1 M) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/dithiothreitol-dtt-1-m.
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