Dithiothreitol (DTT) (1 M) Preparation and Recipe
DTT (Dithiothreitol) is a commonly used reducing agent.
To prepare L of Dithiothreitol (DTT) (1 M):
Change the value in the textbox above to scale the recipe volume
Change the value in the textbox above to scale the recipe volume
Table 1. Required components
Component | Amount | Concentration |
DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) (mw: 154.25 g/mol) | 150 g | 0.9724 M |
- Prepare 800 mL of distilled water in a suitable container.
- Add 150 g of DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) to the solution.
- Add distilled water until the volume is 1 L.
- Dispense into 1-mL aliquots, and store them in the dark (wrapped in aluminum foil) at -20°C (indefinitely). Do not autoclave DTT or solutions containing it.
Physiological Buffer
pH Buffering
Sample Preparation
BioAssays
Misc
Cell/Culture/Growth Media
Gel Electrophoresis
References
This online tool may be cited as follows
MLA | "Quest Calculate™ Dithiothreitol (DTT) (1 M) Preparation and Recipe." AAT Bioquest, Inc., 11 Nov. 2024, https://www.aatbio.com/resources/buffer-preparations-and-recipes/dithiothreitol-dtt-1-m. | |
APA | AAT Bioquest, Inc. (2024, November 11). Quest Calculate™ Dithiothreitol (DTT) (1 M) Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/dithiothreitol-dtt-1-m. | |
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