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Dithiothreitol (DTT) (1 M) Preparation and Recipe

DTT (Dithiothreitol) is a commonly used reducing agent.

To prepare  L of Dithiothreitol (DTT) (1 M):
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) (mw: 154.25 g/mol)150 g0.9724 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 150 g of DTT (DL-dithiothreitol, anhydrous m.w. = 154.25) to the solution.
  3. Add distilled water until the volume is 1 L.
  4. Dispense into 1-mL aliquots, and store them in the dark (wrapped in aluminum foil) at -20°C (indefinitely). Do not autoclave DTT or solutions containing it.

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This online tool may be cited as follows


"Quest Calculate™ Dithiothreitol (DTT) (1 M) Preparation and Recipe." AAT Bioquest, Inc.3 Feb2023


AAT Bioquest, Inc. (2023February 3). Quest Calculate™ Dithiothreitol (DTT) (1 M) Preparation and Recipe. AAT Bioquest.