AAT Bioquest

Anesthetic (MS222) Solution Preparation and Recipe

Anesthetic Solution (Buffered MS222) is commonly used to temporarily immobilize fish and amphibians.

To prepare  L of Anesthetic (MS222) Solution:
Change the value in the textbox above to scale the recipe volume

Table 1. Required components
MS222 (3-aminobenzoic acid ethyl ester; Sigma-Aldrich A5040) (mw: 261.29 g/mol)1 g0.006053 M
Sodium bicarbonate (mw: 84.01 g/mol)0.7 g0.008332 M
  1. Prepare 800 mL of distilled water in a suitable container.
  2. Add 1 g of MS222 (3-aminobenzoic acid ethyl ester; Sigma-Aldrich A5040) to the solution.
  3. Add 0.7 g of Sodium bicarbonate to the solution.
  4. Add distilled water until the volume is 1 L.
  5. The resulting pH should be ~7.0. Store in a foil-covered container at 4°C for up to 1 mo. Warm to room temperature before use.

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Physiological Buffer
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This online tool may be cited as follows


"Quest Calculate™ Anesthetic (MS222) Solution Preparation and Recipe." AAT Bioquest, Inc.1 Mar2024https://www.aatbio.com/resources/buffer-preparations-and-recipes/anesthetic-solution-buffered-ms222.


AAT Bioquest, Inc. (2024March 1). Quest Calculate™ Anesthetic (MS222) Solution Preparation and Recipe. AAT Bioquest. https://www.aatbio.com/resources/buffer-preparations-and-recipes/anesthetic-solution-buffered-ms222.